Expression of Ovol2 is related to epithelial characteristics and shows a favorable clinical outcome in hepatocellular carcinoma.
Expression of Ovol2 is related to epithelial characteristics and shows a favorable clinical outcome in hepatocellular carcinoma.
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Ovol2的表达与上皮特征相关，并在肝细胞癌中显示出良好的临床结果
DOI：
10.2147/ott.s110409
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发表时间：
2016
影响因子：
4
通讯作者：
Guo H
中科院分区：
文献类型：
--
作者：
Fu H;Qi L;Chen L;He Y;Zhang N;Guo H
转移涉及上皮-间充质转化（EMT）和间充质-上皮转化。Ovol 2属于进化上保守的锌指转录因子的Ovo样家族（Ovol），其在各种分化过程中调节基因表达。最近的研究表明，Ovols通过诱导miR-200在一系列人类癌症中的表达来影响间充质-上皮转化。下调的Ovol 2表达参与乳腺癌和前列腺癌的侵袭和转移，但对其在其他癌症（包括肝细胞癌（HCC））中的表达和预后价值知之甚少。本研究旨在探讨Ovol 2在HCC患者中的临床和预后意义。Ovol 2在HCC患者和HCC细胞系的肿瘤样品中的表达使用Western印迹、实时聚合酶链反应和免疫组织化学进行检测。EMT相关标志物的表达水平，包括E-钙粘蛋白，N-钙粘蛋白，和波形蛋白，检测与Ovol 2的表达。Kaplan-Meier和考克斯回归分析Ovol 2在HCC患者中的预后意义。Ovol 2在肝癌组织中的表达明显低于癌旁组织。在组织分化差、微血管浸润和肝硬化的HCC组织中检测到Ovol 2的低表达。在Ovol 2和EMT标记物表达水平之间观察到显著的关系。Kaplan-Meier分析显示，Ovol 2低表达的HCC患者的总生存率显著更差，表明Ovol 2缺失是HCC患者预后不良的独立预测因子。Ovol 2的高表达可能通过抑制EMT抑制HCC细胞的侵袭和转移。
Metastasis involves epithelial–mesenchymal transition (EMT) and mesenchymal–epithelial transition. Ovol2 belongs to the Ovo-like family (Ovol) of evolutionarily conserved zinc-finger transcription factors that regulate gene expression in various differentiation processes. Recent studies have demonstrated that Ovols affect mesenchymal–epithelial transition by inducing the expression of miR-200 in a range of human cancers. Downregulated Ovol2 expression is involved in the invasion and metastasis of breast and prostate cancers, but little is known about its expression and prognostic value in other cancers, including hepatocellular carcinoma (HCC). This study was designed to explore the clinical and prognostic significance of Ovol2 in patients with HCC. The expression of Ovol2 in tumor samples from patients with HCC and HCC cell lines was examined using Western blotting, real-time polymerase chain reaction, and immunohistochemistry. The expression levels of EMT-related markers, including E-cadherin, N-cadherin, and vimentin, were detected in relation to Ovol2 expression. The prognostic significance of Ovol2 in patients with HCC was statistically analyzed by Kaplan–Meier and Cox regression analyses. Ovol2 expression was significantly lower in HCC tissues than in adjacent noncancerous tissues. Low expression of Ovol2 was detected in HCC tissues with poor histological differentiation, microvascular invasion, and cirrhosis. A significant relationship was observed between Ovol2 and EMT marker expression levels. Kaplan–Meier analysis showed that overall survival was significantly worse in patients with HCC with low Ovol2 expression, indicating that Ovol2 deletion was an independent predictor of unfavorable prognosis in patients with HCC. Elevated Ovol2 expression may suppress HCC cell invasion and metastasis via restricting EMT.