Polymorphism of the porcine leptin gene promoter and analysis of its association with gene expression and fatness traits
Polymorphism of the porcine leptin gene promoter and analysis of its association with gene expression and fatness traits
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DOI：
10.1007/s10528-006-9070-x
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发表时间：
2007-04-01
影响因子：
2.4
通讯作者：
Switonski, M.
中科院分区：
文献类型：
--
作者：
Stachowiak, M.;Mackowski, M.;Switonski, M.
我们首次描述了一个245 bp的片段的猪瘦素基因启动子的转录起始位点附近。应用PCR-SSCP技术对720头猪进行了多态性检测。已鉴定出四种SNPs，分离为两种单倍型，其中一种（C113 G）位于AP-2转录因子的推定共有位点。这种多态性均匀分布在杜洛克品种（n=21），波兰长白猪（n=248）和皮特兰品种（n=12）中不存在。在波兰大白色（n=191）和合成系990（n=243）中，等位基因G以非常低的频率出现。研究C113 G SNP是否影响皮下脂肪中瘦素mRNA水平和血清中瘦素蛋白浓度。此外，该多态性对肥满度性状的影响进行了统计分析。虽然GG动物中的表达有降低的趋势，但基因型之间的差异不显著。我们也没有发现LEP启动子基因型与分析的肥胖性状相关的证据。
We describe for the first time a 245 bp fragment of the porcine leptin gene promoter in the proximity of the transcription start site. Altogether, 720 pigs were screened with the PCR-SSCP technique for polymorphism in this region. Four SNPs, segregating as two haplotypes, have been identified, one of them (C113G) in the putative consensus site for the AP-2 transcription factor. This polymorphism was evenly distributed in the Duroc breed (n=21) and was absent in the Polish Landrace (n=248) and Pietrain breed (n=12). In the Polish Large White (n=191) and synthetic line 990 (n=243), allele G occurred with a very low frequency. The investigation was performed to test if the C113G SNP affects leptin mRNA level in subcutaneous fat and leptin protein concentration in serum. Additionally, the effect of this polymorphism on fatness traits was statistically analyzed. Although there was a trend toward decreased expression in GG animals, the differences were not significant between genotypes. We also found no evidence for an association of the LEP promoter genotype with the analyzed fatness traits.