Characterizing the antioxidant activity of amla (Phyllanthus emblica) extract
Characterizing the antioxidant activity of amla (Phyllanthus emblica) extract
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表征余甘子（余甘子）提取物的抗氧化活性
DOI：
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发表时间：
2001
期刊：
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通讯作者：
J. Mittal
中科院分区：
文献类型：
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作者：
S. Khopde;K. Priyadarsini;H. Mohan;V. Gawandi;J. G. Satav;J. Yakhmi;M. Banavaliker;M. Biyani;J. Mittal
Amla以其丰富的维生素C（抗坏血酸）和多酚含量而闻名。为了评估其抗氧化活性，我们检测了amla水提取物对γ辐射诱导的大鼠肝微粒体脂质过氧化（LPO）和大鼠肝线粒体超氧化物歧化酶（SOD）损伤的抑制能力。LPO实验中，加入amla提取物作为水溶液；照射时间间隔不同。用硫代巴比妥酸反应物质测定LPO的程度。结果表明，amla提取物对γ辐射诱导的LPO具有良好的抗氧化作用。同样，发现它可以抑制抗氧化酶SOD的损伤。该提取物的抗氧化活性具有剂量依赖性和浓度依赖性。用高效液相色谱法和滴定法测定了amla中抗坏血酸的含量，测定结果为3.25 ~ 4.5% w/w。然而，在含有这种纯抗坏血酸组成的微粒体中，没有观察到LPO的抑制作用。用循环伏安法测定amla提取物的抗坏血酸当量，发现其为amla的9.4% w/w。当与amla和抗坏血酸对ABTS自由基（一种稳定的自由基）的反应性进行比较时，发现这个值是一致的。基于这些结果，我们得出结论，amla是一种比维生素C更有效的抗氧化剂。
Amla is well-known for its rich vitamin C (ascorbic acid) and polyphenol contents. To assess its antioxidant activity, we examined aqueous amla extract for its ability to inhibit γ-radiation-induced lipid peroxidation (LPO) in rat liver microsomes and superoxide dismutase (SOD) damage in rat liver mitochondria. For the LPO experiment, amla extract was added as its aqueous solution; and irradiation was carried out at different time intervals. The extent of LPO was measured in terms of thiobarbituric acid reactive substances. It was observed that the amla extract acts as a very good antioxidant against γ-radiationinduced LPO. Similarly, it was found to inhibit the damage to antioxidant enzyme SOD. The antioxidant activity of the amla extract was found to be both doseand concentration-dependent. The amount of ascorbic acid in amla was standardized by HPLC and titrimetric methods and was found to be 3.25 to 4.5% w/w. However in microsomes containing this composition of pure ascorbic acid alone, no inhibition in LPO was observed. Cyclic voltammetry of the amla extract was carried out to estimate the ascorbic acid equivalents, which was found to be 9.4% w/w of amla. This value was found to be in agreement when compared with the reactivity of both amla and ascorbic acid towards ABTS radical, a stable free-radical. Based on these results it is concluded that amla is a more potent antioxidant than vitamin C.