Stratified tissue biofabrication by rotational internal flow layer engineering.
Stratified tissue biofabrication by rotational internal flow layer engineering.
复制标题
通过旋转内流层工程进行分层组织生物制造。
DOI：
10.1088/1758-5090/ace2ed
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发表时间：
2023
期刊：
影响因子：
9
通讯作者：
Holland I
中科院分区：
文献类型：
--
作者：
Holland I
紧密模仿人类组织学的分层组织的生物组装对组织工程提出了挑战。现有的生物打印技术缺乏形成通常在分层组织中观察到的微尺度细胞宽度层所需的分辨率和细胞密度，特别是当使用低粘度水凝胶（如胶原蛋白）时。在这里，我们提出了旋转内流层工程（步枪），一种新的，低成本的生物织物技术，用于组装可调的，多层组织样结构。使用高速旋转的管状模具，将添加到内表面的小体积的载有细胞的液体转变成薄层并凝胶化，逐步构建由离散的微米级层组成的宏观管，其厚度是旋转速度的函数。细胞包封使高密度层（10 8个细胞ml− 1）形成异质结构。步枪的多功能性通过图尼卡培养基组装得到证明，将人类平滑肌细胞包裹在细胞宽度（12.5 µm）的胶原蛋白层中。离散的微米级层的这种沉积有助于模仿天然分层组织的性质的复合结构的生物制造。这项技术有可能让研究人员经济地创造出一系列有代表性的分层组织。
The bioassembly of layered tissue that closely mimics human histology presents challenges for tissue engineering. Existing bioprinting technologies lack the resolution and cell densities necessary to form the microscale cell-width layers commonly observed in stratified tissue, particularly when using low-viscosity hydrogels, such as collagen. Here we present rotational internal flow layer engineering (RIFLE), a novel, low-cost biofabrication technology for assembling tuneable, multi-layered tissue-like structures. Using high-speed rotating tubular moulds, small volumes of cell-laden liquids added to the inner surface were transitioned into thin layers and gelled, progressively building macroscale tubes composed of discrete microscale strata with thicknesses a function of rotational speed. Cell encapsulation enabled the patterning of high-density layers (10 8 cells ml− 1) into heterogenous constructs. RIFLE versatility was demonstrated through tunica media assembly, encapsulating human smooth muscle cells in cell-width (12.5 µm) collagen layers. Such deposition of discrete microscale layers, facilitates the biofabrication of composite structures mimicking the nature of native stratified tissue. This enabling technology has the potential to allow researchers to economically create a range of representative layered tissue.