Two capillary approach for a multifunctional nanoflow sheath liquid interface for capillary electrophoresis-mass spectrometry
Two capillary approach for a multifunctional nanoflow sheath liquid interface for capillary electrophoresis-mass spectrometry
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DOI：
10.1002/elps.202000169
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发表时间：
2020-08-19
期刊：
影响因子：
2.9
通讯作者：
Neusuess, Christian
中科院分区：
文献类型：
--
作者：
Hoecker, Oliver;Knierman, Mike;Neusuess, Christian
CE联用ESI-MS（CE-ESI-MS）是一种成熟的分析复杂样品中带电分析物的技术。尽管用于CE-MS耦合的各种接口是商业上可获得的，但是结合联合收割机灵敏度、简单性和鲁棒性的替代品的开发仍然是研究的主题。在这项工作中，一个nanoflow鞘液CE-MS接口与两个可移动的毛细管内的玻璃发射器进行了描述。该设置使分离模式和调节模式分别引导分离毛细管流出物进入电喷雾或废物。这使得能够从MS检测中排除部分分析和到达质谱仪的不需要的基质组分，与LC-MS耦合中的分流阀相当。此外，该功能通过减少阻塞发射器的颗粒来提高系统的整体鲁棒性。即使分离毛细管内置在运行电喷雾的接口中，也可以使用电喷雾干扰物质进行预处理，甚至可以在每次分析中应用涂层材料。通过分析重基质生物反应器样品证明了功能性。总的来说，与以前的方法相比，这种创新提供了更方便的接口安装，改进了处理，延长了发射器尖端的寿命和附加功能，同时保持了纳米流CE-MS耦合的更高灵敏度。
CE hyphenated to ESI-MS (CE-ESI-MS) is a well-established technique to analyze charged analytes in complex samples. Although various interfaces for CE-MS coupling are commercially available, the development of alternatives which combine sensitivity, simplicity, and robustness remains a topic of research. In this work, a nanoflow sheath liquid CE-MS interface with two movable capillaries inside a glass emitter is described. The setup enables aseparation modeand aconditioning modeto guide the separation capillary effluent either into the electrospray or to the waste, respectively. This enables to exclude parts of the analysis from MS detection and unwanted matrix components reaching the mass spectrometer, comparable to divert valves in LC-MS coupling. Also, this function improves the overall robustness of the system by reduction of particles blocking the emitter. Preconditioning with electrospray interfering substances and even the application of coating materials for every analysis is enabled, even while the separation capillary is built into the interface with running electrospray. The functionality is demonstrated by analyses of heavy matrix bioreactor samples. Overall, this innovation offers a more convenient installation of the interface, improved handling with an extended lifetime of the emitter tips and additional functions compared to previous approaches, while keeping the higher sensitivity of nanoflow CE-MS-coupling.