A YEAST MUTANT LACKING MITOCHONDRIAL PORIN IN RESPIRATORY-DEFICIENT, BUT CAN RECOVER RESPIRATION WITH SIMULTANEOUS ACCUMULATION OF AN 86-KD EXTRAMITOCHONDRIAL PROTEIN
A YEAST MUTANT LACKING MITOCHONDRIAL PORIN IN RESPIRATORY-DEFICIENT, BUT CAN RECOVER RESPIRATION WITH SIMULTANEOUS ACCUMULATION OF AN 86-KD EXTRAMITOCHONDRIAL PROTEIN
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DOI：
10.1002/j.1460-2075.1987.tb04813.x
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发表时间：
1987-03-01
期刊：
影响因子：
11.4
通讯作者：
SCHATZ, G
中科院分区：
文献类型：
--
作者：
DIHANICH, M;SUDA, K;SCHATZ, G
通过基因破坏构建了一个缺乏线粒体外膜唯一已知的成孔蛋白的酵母突变体。该突变体保留了线粒体外膜的所有其他主要蛋白质，但严重缺乏线粒体细胞色素，最初不生长的非发酵碳源，甘油。然而，它可以慢慢地适应甘油：适应伴随着部分恢复细胞色素水平和大量积累的86 kd的多肽extramitoditrial细胞组分。
A yeast mutant lacking the only known pore-forming protein of the mitochondrial outer membrane was constructed by gene disruption. The mutant retained all other major proteins of the mitochondrial outer membrane, but was severely deficient in mitochondrial cytochromes and initially did not grow on the non-fermentable carbon source, glycerol. However, it could slowly adapt to glycerol: adapation was accompanied by the partial restoration of cytochrome levels and massive accumulation of an 86-kd polypeptide in extramitochondrial cell fractions.