Development of an adult mouse model for studies on protection against rotavirus
Development of an adult mouse model for studies on protection against rotavirus
复制标题
开发用于研究轮状病毒保护的成年小鼠模型
DOI：
10.1128/jvi.64.10.5070-5075.1990
复制
发表时间：
1990
影响因子：
5.4
通讯作者：
John F. Sheridan
中科院分区：
文献类型：
--
作者：
R. L. Ward;Monica M. McNeal;John F. Sheridan
尽管小鼠已被用作轮状病毒疾病研究的动物模型，但这些研究受到出生后小鼠易患轮状病毒疾病的短时间段（即，约15天）。为了克服这一局限性，开发了一种成年小鼠模型，其中终点是感染而不是疾病。开发的模型利用了一种小鼠轮状病毒（EDIM）株，该病毒株通过在MA 104细胞中多次传代而适应于在培养物中生长。EDIM的第二次细胞培养传代引起新生BALB/c小鼠严重腹泻，并且在9次细胞培养传代后观察到很少或没有疾病改善，即使当该制剂被空斑纯化时。经口给予2 × 10（3）PFU第9代也持续引起4、10、15、30、60、120和180日龄小鼠感染，通过病毒脱落和血清转化测定。在第一次接种后2、3或4个月，用相同的病毒制剂再次接种这些小鼠，没有再感染的证据。相反，用异型WC 3牛轮状病毒感染新生小鼠并不能防止用培养适应的EDIM再感染。因此，该菌株的EDIM引起一致的感染，以前未接种的新生儿和成年BALB/c小鼠，并产生同型，但不异型保护再感染。
Although mice have been used as an animal model for studies on rotavirus disease, these studies have been limited by the short time period after birth during which mice are susceptible to rotavirus illness (i.e., approximately 15 days). To overcome this limitation, an adult mouse model was developed in which the endpoint was infection rather than illness. The model developed utilized a strain of mouse rotavirus (EDIM) adapted to grow in culture by multiple passages in MA104 cells. The second cell culture passage of EDIM caused severe diarrhea in neonatal BALB/c mice, and little or no amelioration of disease was observed after nine cell culture passages, even when this preparation was plaque purified. Oral administration of 2 x 10(3) PFU of passage 9 also consistently caused infection of mice 4, 10, 15, 30, 60, 120, and 180 days of age as determined by viral shedding and seroconversion. Reinoculation of these mice with the same virus preparation at 2, 3, or 4 months after the first inoculation produced no evidence of reinfection. In contrast, infection of neonatal mice with the heterotypic WC3 bovine rotavirus did not prevent reinfection with culture-adapted EDIM. Thus, this strain of EDIM caused consistent infection of previously uninoculated neonatal and adult BALB/c mice and produced homotypic but not heterotypic protection against reinfection.