The isoelectric fractionation of hen's-egg ovotransferrin.
The isoelectric fractionation of hen's-egg ovotransferrin.
复制标题
鸡蛋卵转铁蛋白的等电分离。
DOI：
10.1042/bj1080069
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发表时间：
1968
期刊：
影响因子：
--
通讯作者：
J. Williams
中科院分区：
文献类型：
--
作者：
R. Wenn;J. Williams
1.等电分级法检测母鸡卵转铁蛋白。2.在淀粉凝胶电泳法中观察到的主要成分与次要成分可以分离。3.当向卵转铁蛋白中加入非饱和铁时，等电分离表明存在三种对应于无金属蛋白质的分子物种：一铁原子-蛋白质复合体和两铁原子-蛋白质复合体。4.用~(59)Fe标记的人血清等电分离表明，正常人血清中的转铁蛋白还以无金属蛋白、一铁原子-蛋白质复合体和两铁原子-蛋白质复合体的形式存在。5.得出了第一、第二铁原子的结合常数相似的结论。
1. Hen ovotransferrin was examined by isoelectric fractionation. 2. The major component observed in starch-gel electrophoresis can be isolated from the minor component. 3. When non-saturating amounts of iron are added to ovotransferrin, isoelectric fractionation demonstrates the existence of three molecular species corresponding to the metal-free protein, the one-iron-atom-protein complex and the two-iron-atoms-protein complex. 4. Isoelectric fractionation of human serum labelled with (59)Fe suggests that the transferrin of normal human serum also exists as metal-free protein, the one-iron-atom-protein complex and the two-iron-atoms-protein complex. 5. It is concluded that the binding constants for the first and second iron atoms are similar.