The chemokine receptor CCR7 is expressed on epithelium of non-inflamed gastric mucosa, Helicobacter pylori gastritis, gastric carcinoma and its precursor lesions and up-regulated by H-pylori
The chemokine receptor CCR7 is expressed on epithelium of non-inflamed gastric mucosa, Helicobacter pylori gastritis, gastric carcinoma and its precursor lesions and up-regulated by H-pylori
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DOI：
10.1111/j.1365-2249.2005.02703.x
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发表时间：
2005-02-01
影响因子：
4.6
通讯作者：
Eck, M
中科院分区：
文献类型：
--
作者：
Schmausser, B;Endrich, S;Eck, M
胃癌肿瘤细胞上CCR7趋化因子受体的表达与淋巴结转移相关，并被认为在转移中发挥重要作用。然而，到目前为止，尚不清楚CCR7是在胃癌中新近表达上调还是已经在非肿瘤性胃上皮中表达。因此，研究上皮细胞CCR7在胃癌发生过程中的表达：非炎症粘膜-幽门螺杆菌胃炎-肠上皮化生/不典型增生-胃癌。通过免疫组织化学检测，CCR7 在非炎症胃粘膜 (n = 5)、幽门螺杆菌胃炎 (n = 17)、肠化生 (n = 10)、不典型增生 (n = 3) 的胃上皮以及 24 例胃癌患者中的 20 例肿瘤细胞上表达（13/14 肠型；7/10 弥漫型）。由于H. pylori胃炎中胃上皮的CCR7表达明显强于未感染的粘膜，因此通过荧光激活细胞分选仪分析研究H. pylori对胃上皮细胞CCR7受体表达的影响。幽门螺杆菌菌株上调 CCR7 阳性细胞系中的 CCR7 趋化因子受体。 cag(+) 和 cag(-)H 之间 CCR7 上调没有差异。发现了幽门螺杆菌菌株。幽门螺杆菌上皮细胞 CCR7 的上调可能会改变胃癌的转移命运。此外，CCR7 不仅在胃癌上表达，而且在非肿瘤性胃上皮上表达，表明其具有新的生物学功能。
CCR7 chemokine-receptor expression on tumour cells of gastric carcinoma has been associated with lymph-node metastasis and is thought to play an important role in metastasis. However, so far it is unknown whether CCR7 is newly up-regulated on gastric carcinoma or already expressed in non-neoplastic gastric epithelium. Therefore, epithelial CCR7 expression was investigated in the process of gastric carcinogenesis: non-inflamed mucosa -Helicobacter pylori gastritis - intestinal metaplasia/dysplasia - gastric carcinoma. CCR7 was expressed by gastric epithelium in non-inflamed gastric mucosa (n = 5), H. pylori gastritis (n = 17), intestinal metaplasia (n = 10), dysplasia (n = 3) and on tumour cells in 20 of 24 patients with gastric carcinoma (13/14 intestinal-type; 7/10 diffuse-type) as tested by immunohistochemistry. As CCR7 expression by gastric epithelium was significantly stronger in H. pylori gastritis than in non-infected mucosa, the influence of H. pylori on CCR7 receptor expression of gastric epithelial cells was investigated by fluorescence activated cell sorter analysis. H. pylori strains up-regulated the CCR7 chemokine-receptor in CCR7-positive cell lines. No difference in CCR7 up-regulation between cag(+) and cag(-)H. pylori strains was found. Epithelial CCR7 up-regulation by H. pylori may alter the metastatic fate of gastric carcinoma. Additionally, CCR7 expression not only on gastric carcinoma, but also on non-neoplastic gastric epithelium, suggests a novel biological function.