Possible enhancing effects of atrazine and nonylphenol on 7,12-dimethylbenz[a]anthracene-induced mammary tumor development in human c-Ha-ras proto-oncogene transgenic rats
Possible enhancing effects of atrazine and nonylphenol on 7,12-dimethylbenz[a]anthracene-induced mammary tumor development in human c-Ha-ras proto-oncogene transgenic rats
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DOI：
10.1111/j.1349-7006.2004.tb03223.x
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发表时间：
2004-05-01
期刊：
影响因子：
5.7
通讯作者：
Tsuda, H
中科院分区：
文献类型：
--
作者：
Fukamachi, K;Han, BS;Tsuda, H
我们的转基因（Tg）菌株携带拷贝的人类c-Ha-ras原癌基因是高度敏感的7，12-二甲基苯并[a]蒽（DMBA）诱导的乳腺癌发生，可能是由于激活的转基因，并可用于中期生物测定模型，以测试修改影响雌激素环境化合物对肿瘤的发展。本研究旨在评估饮食喂养的内分泌干扰物阿特拉津和壬基酚对DMBA诱导的c-Ha-ras Tg大鼠致癌作用的影响。雄性和雌性动物在50日龄时给予单次口服剂量的DMBA（25 mg/kg体重），此后接受含有5、50或500 ppm阿特拉津或10、25、100或250 ppm壬基酚的无大豆饮食。在雌性Tg大鼠中，5 ppm剂量的阿特拉津增加了乳腺腺瘤和腺癌的发病率（P
Our transgenic (Tg) strain carrying copies of the human c-Ha-ras proto-oncogene is highly susceptible to 7,12-dimethylbenz[a]anthracene (DMBA)-induced mammary carcinogenesis, possibly due to activation of the transgene, and can be used in medium-term bioassay models to test for modifying effects of estrogenic environmental compounds on tumor development. The present study was conducted to assess the influence of dietary feeding of the endocrine disruptors atrazine and nonylphenol on DMBA-induced carcinogenesis in c-Ha-ras Tg rats. Animals of both sexes were given a single oral dose of DMBA (25 mg/kg body weight) at 50 days of age and thereafter received soybean-free diet containing 5, 50 or 500 ppm atrazine, or 10, 25, 100 or 250 ppm nonylphenol. In female Tg rats, atrazine at a dose of 5 ppm increased the incidences of mammary adenomas and adenocarcinomas (P