MicroRNA profiles to predict postoperative prognosis in patients with small cell carcinoma of the esophagus.
MicroRNA profiles to predict postoperative prognosis in patients with small cell carcinoma of the esophagus.
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发表时间：
2015-02
影响因子：
2
通讯作者：
T. Okumura;Y. Shimada;T. Omura;K. Hirano;T. Nagata;K. Tsukada
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作者：
T. Okumura;Y. Shimada;T. Omura;K. Hirano;T. Nagata;K. Tsukada
背景/目的食道小细胞癌(SCCE)是一种少见的侵袭性疾病，预后差。本研究的目的是确定一个分子标记来预测SCCE患者的术后结果。材料与方法应用微阵列技术检测手术切除的SCCE肿瘤组织中microRNA的表达。建立了SCCE细胞系(TYUC-1)，以研究差异表达的microRNAs的生物学作用。结果microRNA表达的分级聚类法显示，肿瘤快速复发(n=3，中位生存期5.1月)和长期生存(n=3，中位观察，144.7个月)的两个离散聚类分别相同。8个microRNAs(miR-4323、miR-625、miR-3619-3p、miR-4419b、miR-1249、miR-4648、miR-4664-3p和miR-1203)与肿瘤复发显著相关(p<；0.01)。MiR-625下调可显著抑制TYUC-1的迁移。结论肿瘤组织中microRNAs的表达谱可作为预测SCCE患者术后预后的新指标。
BACKGROUND/AIM Small cell carcinoma of the esophagus (SCCE) is a rare but very aggressive disease with poor prognosis. The aim of the present study was to identify a molecular signature to predict postoperative outcomes in patients with SCCE. MATERIALS AND METHODS Expression of microRNA was detected in surgically-removed SCCE tumors using microarrays. A SCCE cell line (TYUC-1) was established to investigate the biological role of differentially expressed microRNAs. RESULTS Hierarchical clustering of microRNA expression revealed two discrete clusters that were identical to the cases with rapid tumor relapse (n=3; median survival, 5.1 months) and the cases with long-term survival (n=3; median observation, 144.7 months), respectively. Eight microRNAs (miR-4323, miR-625, miR-3619-3p, miR-4419b, miR-1249, miR-4648, miR-4664-3p and miR-1203) showed significant correlation with tumor relapse (p<0.01). Migration of TYUC-1 was significantly inhibited by down-regulation of miR-625. CONCLUSION The expression profiles of microRNAs in tumors may represent a novel predictor for postoperative outcomes in patients with SCCE.