Conjugated Polymer-Based Photoelectrochemical Cytosensor with Turn-On Enable Signal for Sensitive Cell Detection
Conjugated Polymer-Based Photoelectrochemical Cytosensor with Turn-On Enable Signal for Sensitive Cell Detection
复制标题
具有开启使能信号的共轭聚合物光电化学细胞传感器，用于敏感细胞检测
DOI：
10.1021/acsami.7b18275
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发表时间：
2018-02-21
影响因子：
9.5
通讯作者：
Wang, Shu
中科院分区：
文献类型：
--
作者：
Liu, Shanshan;He, Ping;Wang, Shu
本文以阳离子型聚芴衍生物聚（9，9-双（6 '-（N，N，N，-三甲基铵）己基）芴-co-alt-1，4-亚苯基）溴化物（PFP）为光电响应材料，构建了一种新型光电化学细胞传感器。带正电荷的PFP具有很高的光电转换效率，在光照下可以产生很强的光电流。在PEC细胞传感器中，3-膦酰基丙酸连接到氧化铟锡电极，然后通过酰胺缩合反应与抗上皮细胞粘附分子（EpCAM）抗体修饰。因此，具有过表达EpCAM抗原的靶SKBR-3细胞可以通过特异性抗体抗原相互作用被捕获到电极上。在加入阳离子PFP后，阳离子PFP与带负电荷的细胞膜之间有利的静电相互作用导致靶SKBR-3细胞的开启检测信号。这种新型的细胞传感器不仅由于共轭聚合物良好的光电性能而表现出良好的灵敏度，而且由于利用了特异性抗体抗原识别，对靶细胞具有良好的选择性。
In this work, a new photoelectrochemical (PEC) cytosensor was constructed by using cationic polyfluorene derivative, poly(9,9-bis(6'-(N,N,N,-trirnethylammonium)hexyl)fluorene-co-alt-1,4-phenylene)bromide (PFP) as the photoelectric-responsive material for sensitive cell detection. Positive-charged PFP with high photoelectric conversion efficiency can generate robust photocurrent under light illumination. In the PEC cytosensor, 3-phosphonopropionic acid was linked to the indium tin oxide electrode, followed by modification with antiepithelial-cell-adhesion-molecule (EpCAM) antibody via amide condensation reaction. Thus, target SKBR-3 cells with overexpressed EpCAM antigen could be captured onto the electrode via the specific antibody antigen interactions. Upon adding cationic PFP, a favorable electrostatic interaction between cationic PFP and negatively charged cell membrane led to a turn-on detection signal for target SKBR-3 cells. This new cytosensor not only exhibits good sensitivity because of the good photoelectric performance of conjugated polymers, but also offers decent selectivity to target cells by taking advantage of the specific antibody antigen recognition.