DIFFERENTIAL ACTIVATION OF XENOPUS HOMEO BOX GENES BY MESODERM-INDUCING GROWTH-FACTORS AND RETINOIC ACID
DIFFERENTIAL ACTIVATION OF XENOPUS HOMEO BOX GENES BY MESODERM-INDUCING GROWTH-FACTORS AND RETINOIC ACID
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DOI：
10.1101/gad.4.11.1910
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发表时间：
1990-11-01
影响因子：
10.5
通讯作者：
DEROBERTIS, EM
中科院分区：
文献类型：
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作者：
CHO, KWY;DEROBERTIS, EM
胚胎发生过程中位置信息的本质是什么？通过将非洲爪蟾同源框基因用作前后（A - P）标记，我们证实了其他人的发现，即中胚层诱导生长因子和视黄酸（RA）能够沿身体轴线提供位置信息。非洲爪蟾组织培养中胚层诱导因子（XTC - MIF）选择性地激活一个在前部表达的同源框基因（X1Hbox 1），而碱性成纤维细胞生长因子（bFGF）选择性地激活一个在后部表达的同源框基因（X1Hbox 6）。视黄酸激活后部基因X1Hbox 6的表达，但不激活X1Hbox 1。然而，这种激活需要暴露于生长因子。这些数据表明，在脊椎动物中，生长因子和视黄酸可能相互协作以提供位置信息。
What is the nature of positional information during embryogenesis? By using Xenopus homeo box genes as anteroposterior (A-P) markers, we confirm the findings of others that mesoderm-inducing growth factors and retinoic acid (RA) can provide positional information along the axis of the body. Xenopus tissue culture-mesoderm-inducing factor (XTC-MIF) selectively activates an anteriorly expressed homeo box gene (X1Hbox 1), while basic fibroblast growth factor (bFGF) activates selectively a posteriorly expressed homeo box gene (X1Hbox 6). RA activates expression of the posterior gene X1Hbox 6, but not of X1Hbox 1. This activation, however, requires exposure to growth factors. The data suggest that growth factors and RA may cooperate with each other to provide positional information in vertebrates.