Population development within the coffee wilt pathogen Gibberella xylarioides reflects host-related divergence
Population development within the coffee wilt pathogen Gibberella xylarioides reflects host-related divergence
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DOI：
10.1007/s10658-015-0613-z
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发表时间：
2015-06-01
影响因子：
1.8
通讯作者：
Bridge, P. D.
中科院分区：
文献类型：
--
作者：
Buddie, A. G.;Crozier, J.;Bridge, P. D.
通过部分翻译延伸因子基因测序、富含A + T的DNA限制性片段长度多态性（RFLP）分析和简单重复序列聚合酶链指纹分析，对45株分离自非洲咖啡树的木状赤霉（Gibberella xylarioides）培养物的变异进行了评估。结果进行了分析，在宿主植物物种和交配相容性确定的生物物种（BS）。此外，20世纪90年代以前分离的5个“历史”菌株被认为属于G. xylarioides，从国际公认的培养物保藏中心获得，另外10个各种赤霉属/镰刀菌属的菌株，分离自咖啡属，包括在内，以提供上下文比较。对建议的BS的代表进行核糖体基因间间隔区（IGS）测序。结果证实所有菌株均为G.在一些实施方案中，不育群4（SG 4）的菌株可能代表一个或多个另外的“隐蔽”物种。ISSR指纹图谱显示单一宿主相关的差异，表明稳定的克隆传播，这是支持一致的A + T丰富的DNA带型的所有菌株。相反，IGS序列鉴定了与BS相关的潜在系统发育事件。
Variation of 45 Gibberella xylarioides cultures isolated from coffee trees in Africa was assessed by sequencing the partial translation elongation factor gene, by A + T rich DNA restriction fragment length polymorphism (RFLP) analysis and by inter-simple sequence repeat polymerase chain fingerprinting. Results were analysed in relation to host plant species and mating compatibilities to determined biological species (BS). Additionally, five "historical" strains isolated prior to the 1990s, and considered to belong to G. xylarioides, were obtained from internationally-recognised culture collections and a further 10 strains of various Gibberella/Fusarium spp., isolated from Coffea spp., were included to provide contextual comparisons. Ribosomal intergenic spacer (IGS) sequencing was undertaken for representatives of the suggested BS. Results confirmed all strains as G. xylarioides and that strains of sterile group 4 (SG4) may represent one or more additional "cryptic" species. ISSR fingerprints showed a single host correlated difference, suggesting stable clonal spread and this was supported by consistent A + T rich DNA band patterns for all strains. In contrast the IGS sequences identified potential phylogenetic events correlated to BS.