THE IMPRINTED H19 GENE AS A TUMOR-MARKER IN BLADDER-CARCINOMA
THE IMPRINTED H19 GENE AS A TUMOR-MARKER IN BLADDER-CARCINOMA
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DOI：
10.1016/0090-4295(95)80030-1
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发表时间：
1995-02-01
期刊：
影响因子：
2.1
通讯作者：
HOCHBERG, A
中科院分区：
文献类型：
--
作者：
ARIEL, I;LUSTIG, O;HOCHBERG, A
目标.基因组印记是一种新发现的与肿瘤发生有关的遗传学机制。H19是人类中的印记基因，由母体等位基因表达，其在胎儿生命中广泛转录，但不翻译并作为RNA分子发挥功能。它已被认为是一个候选的肿瘤抑制基因。我们研究了H19在胎儿期表达H19的组织中产生的人类癌症中的表达，其中之一是膀胱粘膜。原位杂交检测不同组织学分级膀胱癌石蜡切片中H19 mRNA的表达。低级别（1/3级），非浸润性（Ta）乳头状膀胱移行细胞癌不表达H19，但在较高级别，浸润性膀胱移行细胞癌（T1-T3/4）的显着表达。原位膀胱癌（Tis）中的表达也很明显，Tis倾向于迅速进展为浸润性癌。我们认为，H19可以作为一种肿瘤标记物在人类膀胱癌，它的表达表明一个更恶性的潜力。
Objectives. Genomic imprinting is a newly discovered mechanism in genetics that is involved in tumorigenesis. H19 is an imprinted gene in the human, expressed from the maternal allele, It is extensively transcribed in fetal life but is not translated and functions as an RNA molecule. It has been suggested as a candidate tumor suppressor gene. We studied the expression of H19 in human cancer arising from tissues expressing H19 in fetal life, one of which is bladder mucosa.Methods. in situ hybridization for H19 mRNA on paraffin sections of bladder carcinoma in different histologic grades.Results. Low-grade (grade 1 of 3), noninvasive (Ta) papillary transitional cell bladder carcinoma did not express H19, but prominent expression was disclosed in higher grades, invasive transitional cell carcinomas (T1-T3/4). Expression was also evident in in situ bladder carcinoma (Tis), which tends to progress rapidly to invasive cancer.Conclusions. We suggest that H19 can be used as a tumor marker in human bladder carcinoma, where its expression indicates a more malignant potential.