Cell motion and recovery in a two-stream microfluidic device
Cell motion and recovery in a two-stream microfluidic device
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DOI：
10.1007/s10404-009-0470-1
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发表时间：
2010-04-01
影响因子：
2.8
通讯作者：
Hubel, Allison
中科院分区：
文献类型：
--
作者：
Mata, Clara;Longmire, Ellen;Hubel, Allison
设计了一种双流微流控装置，用于从细胞悬浮液中提取冷冻保护剂，在一系列条件下测试了细胞的运动。Jurkat细胞（淋巴母细胞）在10%二甲亚砜溶液中与磷酸盐缓冲盐水溶液冲洗流平行驱动，通过单个矩形通道切片和串联多个切片。观察细胞流速率和细胞体积分数（CVF）对细胞活力和恢复的影响。通道深度为500 μ m，通道内细胞流平均流速为3.6 ~ 8.5 mm/s，细胞流雷诺数为2.6 ~ 6.0。在所有检查的病例中，在设备出口测量的细胞活力都很高，表明设备内没有明显的细胞损伤。在单级的下游，平均细胞流速度为千分之一/ 6毫米/秒，细胞回收率为90-100%，而CVFs高达20%。在一个关键的设备填充时间后，多级设备下游的细胞回收率也达到90-100%。这个时间是电池在设备内平均停留时间的五倍。测量的回收率值明显大于使用常规细胞洗涤方法通常获得的回收率值。
The motion of cells in a two-stream microfluidic device designed to extract cryoprotective agents from cell suspensions was tested under a range of conditions. Jurkat cells (lymphoblasts) in a 10% dimethylsulfoxide solution were driven in parallel with phosphate-buffered saline solution wash streams through single rectangular channel sections and multiple sections in series. The influence of cell-stream flow rate and cell volume fraction (CVF) on cell viability and recovery were examined. The channel depth was 500 mu m, and average cell stream velocity within the channels was varied from 3.6 to 8.5 mm/s corresponding with cell stream Reynolds numbers of 2.6-6.0. Cell viability measured at device outlets was high for all cases examined indicating no significant cell damage within the device. Downstream of a single stage, cell recoveries measured 90-100% for average cell stream velocities a parts per thousand yen6 mm/s and for CVFs up to 20%. Cell recovery downstream of multistage devices also measured 90-100% after a critical device population time. This time was found to be five times the average cell residence time within the device. The measured recovery values were significantly larger than those typically obtained using conventional cell washing methods.