Sensitive biosensor array using surface plasmon resonance on metallic nanoslits
Sensitive biosensor array using surface plasmon resonance on metallic nanoslits
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DOI：
10.1117/1.2772296
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发表时间：
2007-07-01
影响因子：
3.5
通讯作者：
Wei, Pei-Kuen
中科院分区：
文献类型：
--
作者：
Lee, Kuang-Li;Lee, Chia-Wei;Wei, Pei-Kuen
制造并测试了用于无标记和高通量检测的基于芯片的生物传感器阵列。该传感器阵列由 150 nm 厚、50 nm 间隙和 600 nm 周期的金纳米狭缝组成。每个阵列尺寸为100μm×100μm。这些金属纳米结构中的横向磁偏振波在水环境中产生波长约 800 nm 的共振表面等离子体。利用纳米狭缝阵列中的共振波长偏移，我们实现了每折射率单位高达 668 nm 的检测灵敏度，大约比纳米孔阵列报道的灵敏度高 1.7 倍。水环境中的抗原-抗体相互作用实验验证了表面结合事件的敏感性。 (C) 2007 年光电仪器工程师协会。
Chip-based biosensor arrays for label-free and high-throughput detection were fabricated and tested. The sensor array was composed of a 150-nm-thick, 50-nm-gap, and 600-nm-period gold nanoslits. Each array size was 100 mu m x 100 mu m. A transverse-magnetic polarized wave in these metallic nanostructures generated resonant surface plasmons at a wavelength of about 800 nm in a water environment. Using the resonant wavelength shift in the nanoslit array, we achieved detection sensitivity up to 668 nm per refractive index unit, about 1.7 times larger than that reported on an array of nanoholes. An antigen-antibody interaction experiment in an aqueous environment verified the sensitivity in a surface binding event. (C) 2007 Society of Photo-Optical Instrumentation Engineers.