STRUCTURE, CHROMOSOMAL LOCATION, AND EXPRESSION PATTERN OF 3 MOUSE GENES HOMOLOGOUS TO THE HUMAN MAGE GENES
STRUCTURE, CHROMOSOMAL LOCATION, AND EXPRESSION PATTERN OF 3 MOUSE GENES HOMOLOGOUS TO THE HUMAN MAGE GENES
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DOI：
10.1006/geno.1995.1108
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发表时间：
1995-07-01
期刊：
影响因子：
4.4
通讯作者：
BOON, T
中科院分区：
文献类型：
--
作者：
DEBACKER, O;VERHEYDEN, AM;BOON, T
人MAGE 1基因指导自体溶细胞性T淋巴细胞识别黑素瘤上的抗原的表达。MAGE 1属于在多种组织学类型的肿瘤中表达，但在除睾丸外的正常组织中不表达的基因家族。IMAGE基因排列成两组，位于人类X染色体的两个不同区域内（Xq 26-qter和Xp21.3），通过将小鼠基因组文库与MAGE 1探针杂交，我们鉴定了三个同源基因，这些小鼠基因中的两个，Smage 1和Smage 2的同源性在99%以上，编码330个氨基酸的相同蛋白。Smage 2的5'端非编码区通过位于第一外显子前的几个不同的启动子提供了调节基因表达的潜力。第三个基因Smage 3具有加工转录物的结构。它编码的蛋白质相对于Smage 1/2产物只有11个氨基酸取代。体细胞杂交和种间回交分析表明，Smage 3是常染色体的，Smage 1和Smage 2位于小鼠X染色体上的Dmd和Ar位点之间，由于该区域与人类Xp21.1-p22.1区域同线，我们推断Smage 1和Smage 2与IMAGE-Xp而不是MAGE-Xq基因同源。Smage 1/2在小鼠肿瘤细胞和胚胎细胞中均有表达，但在正常小鼠组织中除睾丸外均无表达。Smage 3在胚胎发育第11天至第15天均有表达。(C)出版社：Academic Press
The human MAGE1 gene directs the expression of an antigen recognized on a melanoma by autologous cytolytic T lymphocytes. MAGE1 belongs to a family of genes that are expressed in a number of tumors of various histological types but not in normal tissues except testis, The IMAGE genes are arranged in two groups that are located within two different regions of the human X chromosome (Xq26-qter and Xp21.3), By hybridizing mouse genomic libraries with a MAGE1 probe, we identified three homologous genes, Two of these mouse genes, Smage1 and Smage2 are more than 99% identical to each other and encode the same protein of 330 aa. The 5' noncoding region of Smage2 provides the potential for regulating the expression of the gene through several different promoters located in front of alternative first exons. The third gene, Smage3, has the structure of a processed transcript. It codes for a protein with only 11 aa substitutions with respect to the Smage1/2 product. Somatic cell hybrids and interspecific backcross analysis showed that Smage3 is autosomal and that Smage1 and Smage2 are located between the Dmd and the Ar loci on the mouse X chromosome, Since this region is syntenic to the human Xp21.1-p22.1 region, we conclude that Smage1 and Smage2 are homologous to the IMAGE-Xp rather than to the MAGE-Xq genes. Smage1/2 transcripts were detected in several tumor and embryonal cell lines but not in normal mouse tissues with the exception of testis, Expression of Smage3 was found in embryos from Day 11 to Day 15. (C) 1995 Academic Press, Inc.