Ultrasound-mediated structural changes in cells revealed by FTIR spectroscopy: A contribution to the optimization of gene and drug delivery
Ultrasound-mediated structural changes in cells revealed by FTIR spectroscopy: A contribution to the optimization of gene and drug delivery
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DOI：
10.1016/j.saa.2011.08.074
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发表时间：
2011-12-15
影响因子：
4.4
通讯作者：
Castellano, Agostina Congiu
中科院分区：
文献类型：
--
作者：
Grimaldi, Paola;Di Giambattista, Lucia;Castellano, Agostina Congiu
超声波对生物样品的影响越来越受到人们的关注，尤其是在药物和基因的细胞内安全有效地传递方面。未来在这一领域的进展将需要更好地了解超声波和声空化如何影响生物系统的特性。超声辐射引起的细胞形态变化已被广泛研究，但对细胞结构变化的研究较少。我们将目前用于治疗的两种不同的细胞系Jurkat T淋巴细胞和NIH-3T3成纤维细胞分别作为细胞凋亡和基因治疗研究的模型。用傅里叶变换红外光谱(FTIR)作为探针，揭示了主要生物体系中特定分子基团的结构变化。通过胞质分裂阻断微核(CBMN)分析确定了超声对细胞的遗传毒性损伤。FTIR光谱结果结合多元统计分析，针对两种细胞系的所有细胞成分(脂类、蛋白质、核酸)，显示Jurkat细胞在脂类和蛋白质区域对治疗性超声波更敏感，而NIH-3T3细胞在核酸区域更敏感：两种细胞仅在较长时间内存在显著的遗传毒性效应，而在Jurkat细胞中，长时间的超声波暴露也显示出显著的细胞毒性效应。(C)2011爱思唯尔B.V.保留所有权利。
Ultrasound effects on biological samples are gaining a growing interest concerning in particular, the intracellular delivery of drugs and genes in a safe and in a efficient way. Future progress in this field will require a better understanding of how ultrasound and acoustic cavitation affect the biological system properties. The morphological changes of cells due to ultrasound (US) exposure have been extensively studied, while little attention has been given to the cells structural changes.We have exposed two different cell lines to 1 MHz frequency ultrasound currently used in therapy, Jurkat T-lymphocytes and NIH-3T3 fibroblasts, both employed as models respectively in the apoptosis and in the gene therapy studies. The Fourier Transform Infrared (FTIR) Spectroscopy was used as probe to reveal the structural changes in particular molecular groups belonging to the main biological systems. The genotoxic damage of cells exposed to ultrasound was ascertained by the Cytokinesis-Block Micronucleus (CBMN) assay.The FTIR spectroscopy results, combined with multivariate statistical analysis, regarding all cellular components (lipids, proteins, nucleic acids) of the two cell lines, show that Jurkat cells are more sensitive to therapeutic ultrasound in the lipid and protein regions, whereas the NIH-3T3 cells are more sensitive in the nucleic acids region: a meaningful genotoxic effect is present in both cell lines only for long sonication times while in the Jurkat cells also a significant cytotoxic effect is revealed for long times of exposure to ultrasound. (C) 2011 Elsevier B.V. All rights reserved.