Unique constitution of photosystem I with a novel subunit in the cyanobacterium Gloeobacter violaceus PCC 7421
Unique constitution of photosystem I with a novel subunit in the cyanobacterium Gloeobacter violaceus PCC 7421
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DOI：
10.1016/j.febslet.2004.11.013
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发表时间：
2004-12-17
期刊：
影响因子：
3.5
通讯作者：
Mimuro, M
中科院分区：
文献类型：
--
作者：
Inoue, H;Tsuchiya, T;Mimuro, M
采用Tricine-urea-SDS-PAGE、肽质量指纹图谱分析和N-末端测序等方法研究了从蓝细菌Gloeetum violaceus PCC 7421中分离的光系统I复合物的组成。从基因组序列中鉴定出8个亚基（PsaA、PsaB、PsaC、PsaD、PsaE、PsaF、PsaL和PsaM）。发现了一个新的亚基（PsaZ），但PsaI、PsaJ、PsaK和PsaX缺失。PsaB除了保守区之外还具有155个氨基酸的C-末端延伸，并且该结构域类似于肽聚糖结合结构域。这些结果表明，G.紫罗兰具有独特的结构特性。(C)2004年由Elsevier B. V.代表欧洲生物化学学会联合会出版。
Constitution of the photosystem I complex isolated from the cyanobacterium Gloeobacter violaceus PCC 7421 was investigated by tricine-urea-SDS-PAGE, followed by peptide mass fingerprinting or N-terminal sequencing. Eight subunits (PsaA, PsaB, PsaC, PsaD, PsaE, PsaF, PsaL and PsaM) were identified as predicted from the genome sequence. A novel subunit (PsaZ) was discovered, but PsaI, PsaJ, PsaK and PsaX were absent. PsaB has a C-terminal extension with 155 amino acids in addition to the conserved region and this domain is similar to the peptidoglycan-binding domain. These results suggest that PS I complexes of G. violaceus have unique structural properties. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.