REG I enhances chemo- and radiosensitivity in squamous cell esophageal cancer cells
REG I enhances chemo- and radiosensitivity in squamous cell esophageal cancer cells
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DOI：
10.1111/j.1349-7006.2008.00980.x
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发表时间：
2008-12-01
期刊：
影响因子：
5.7
通讯作者：
Sugiyama, Toshihiro
中科院分区：
文献类型：
--
作者：
Hayashi, Kaori;Motoyama, Satoru;Sugiyama, Toshihiro
非常需要鉴定化学和放射敏感性的可靠标记物以及增强鳞状食管癌细胞对抗癌治疗敏感性的关键分子。为了测试再生基因（REG）I的表达是否增强食管鳞状细胞癌细胞的化疗和放射敏感性，我们使用MTT（3-[4,5-二甲基噻唑-2-基]-2,5-二苯基溴化四唑）测定来比较未转染的TE-5和TE-9细胞与稳定转染REG I的细胞的化疗和放射敏感性阿尔法和我贝塔。然后我们使用流式细胞术来确定 REG I 表达是否改变细胞周期进程。在未转染的 TE-5 和 TE-9 细胞中未检测到 REG I mRNA 或蛋白质。 REG I alpha 和 I beta 转染导致 TE-5 和 TE-9 细胞中 REG I mRNA 和蛋白质的强烈表达，进而导致化疗和放射敏感性显着增加。细胞周期进程不受 REG I 表达的影响。因此，REG I 似乎增强了鳞状食管癌细胞的化学和放射敏感性，这表明它可能是改善食管鳞状细胞癌患者的个体化治疗的有用靶点。 （癌症科学 2008 年；99：2491-2495）。
Identification of reliable markers of chemo- and radiosensitivity and the key molecules that enhance the susceptibility of squamous esophageal cancer cells to anticancer treatments would be highly desirable. To test whether regenerating gene (REG) I expression enhances chemo- and radiosensitivity in esophageal squamous cell carcinoma cells, we used MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assays to compare the chemo- and radiosensitivities of untransfected TE-5 and TE-9 cells with those of cells stably transfected with REG I alpha and I beta. We then used flow cytometry to determine whether REG I expression alters cell cycle progression. No REG I mRNA or protein were detected in untransfected TE-5 and TE-9 cells. Transfection with REG I alpha and I beta led to strong expression of both REG I mRNA and protein in TE-5 and TE-9 cells, which in turn led to significant increases in both chemo- and radiosensitivity. Cell cycle progression was unaffected by REG I expression. REG I thus appears to enhance the chemo- and radiosensitivity of squamous esophageal cancer cells, which suggests that it may be a useful target for improved and more individualized treatments for patients with esophageal squamous cell carcinoma. (Cancer Sci 2008; 99: 2491-2495).