Regulated expression of homeobox genes Msx-1 and Msx-2 in mouse mammary gland development suggests a role in hormone action and epithelial-stromal interactions
Regulated expression of homeobox genes Msx-1 and Msx-2 in mouse mammary gland development suggests a role in hormone action and epithelial-stromal interactions
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DOI：
10.1006/dbio.1996.0168
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发表时间：
1996-07-10
影响因子：
2.7
通讯作者：
Daniel, CW
中科院分区：
文献类型：
--
作者：
Friedmann, Y;Daniel, CW
小鼠同源异型盒基因Msx-1和Msx-2与果蝇msh基因相关，并在小鼠胚胎发育过程中在多种组织中表达。我们现在报告发育调节Msx-1和Msx-2在小鼠乳腺中的表达，并表明它们的表达模式指向重要的功能作用。Msx-1和Msx-2的转录本存在于腺的处女小鼠和腺的小鼠在怀孕早期，但转录显着下降，在怀孕后期。低水平的Msx-1转录检测到腺体从泌乳动物和退化的第一天，而Msx-2的表达没有检测到在哺乳期或早期退化。这两个基因的表达逐渐增加退化的进展。Msx-2，但不是Msx-1的表达减少卵巢切除术后或暴露于抗雌激素直接植入腺体。Msx-2表达的激素调节被证实时，成绩单恢复到正常水平后，给予雌激素卵巢切除的动物。Msx-1的原位分子杂交显示转录本定位于乳腺上皮，而Msx-2的表达仅限于导管周围基质。乳腺上皮细胞已被删除的乳腺基质没有转录可检测量的Msx-2，表明表达是由连续的乳腺上皮细胞，并表明这些同源异型盒基因在乳腺发育过程中的间充质-上皮细胞相互作用的作用。(C)出版社：Academic Press，Inc.
The murine homeobox genes Msx-1 and Msx-2 are related to the Drosophila msh gene and are expressed in a variety of tissues during mouse embryogenesis. We now report the developmentally regulated expression of Msx-1 and Msx-2 in the mouse mammary gland and show that their expression patterns point toward significant functional roles. Msx-1 and Msx-2 transcripts were present in glands of virgin mice and in glands of mice in early pregnancy, but transcripts decreased dramatically during late pregnancy. Low levels of Msx-1 transcripts were detected in glands from lactating animals and during the first days of involution, whereas Msx-2 expression was not detected during lactation or early involution. Expression of both genes increased gradually as involution progressed. Msx-2 but not Msx-1 expression was decreased following ovariectomy or following exposure to anti-estrogen implanted directly into the gland. Hormonal regulation of Msx-2 expression was confirmed when transcripts returned to normal levels after estrogen was administered to ovariectomized animals. In situ molecular hybridization for Msx-1 showed transcripts localized to the mammary epithelium, whereas Msx-2 expression was confined to the periductal stroma. Mammary stroma from which mammary epithelium had been removed did not transcribe detectable amounts of Msx-2, showing that expression is regulated by contiguous mammary epithelium, and indicating a role for these homeobox genes in mesenchymal-epithelial interactions during mammary development. (C) 1996 Academic Press, Inc.