Pronociceptive effects of the neuropeptide, nociceptin, in the land snail, Cepaea nemoralis
Pronociceptive effects of the neuropeptide, nociceptin, in the land snail, Cepaea nemoralis
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DOI：
10.1016/0196-9781(96)00105-2
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发表时间：
1996-01-01
期刊：
影响因子：
3
通讯作者：
PerrotSinal, TS
中科院分区：
文献类型：
--
作者：
Kavaliers, M;PerrotSinal, TS
最近从大鼠脑中分离的肽Phe-Gly Phe-Thr-Gly-Ala-Arg-Lys-Ser-Ala-Arg-Lys-Leu-Ala-Asn-Gln-OH被认为是孤儿阿片样受体（ORL（1））的内源性激动剂。这种肽被称为“伤害感受素”（或“痛敏素FQ”），已被认为具有原伤害感受、痛觉过敏的功能。本研究探讨了伤害感受素厌恶性热（伤害性）反应的无脊椎动物，土地蜗牛，Cepaea nemoralis的影响。孤啡肽在Cepaea中具有显著的、剂量相关的促伤害感受作用，而阿片肽强啡肽A与孤啡肽具有一些序列相似性，具有显著的抗伤害感受作用。强啡肽的作用被阻断的κ-阿片受体拮抗剂，nor-binaltorphimine，而那些nociceptin不受影响。每天重复施用伤害感受素导致原伤害感受效应降低，提示对这种阿片样物质相关肽的痛觉过敏作用的耐受性的发展。这些研究结果表明，新的肽，痛敏素，可以影响蜗牛，Cepaea的伤害性反应，在啮齿类动物的方式类似。
The peptide, Phe-Gly Phe-Thr-Gly-Ala-Arg-Lys-Ser-Ala-Arg-Lys-Leu-Ala-Asn-Gln-OH, recently isolated from rat brain, has been suggested to be an endogenous agonist for an orphan, opioid-like receptor (ORL(1)). This peptide, called ''nociceptin'' (or orphanin FQ), has been su,ggested to have pronociceptive, hyperalgesic functions. The present study examined the effects of nociceptin aversive thermal (nociceptive) responses in an invertebrate, the land snail, Cepaea nemoralis. Nociceptin had significant, dose-related pro-nociceptive effects in Cepaea, whereas the opioid peptide, dynorphin A, which shares some sequence similarities with nociceptin, had significant antinociceptive effects. The effects of dynorphin were blocked by the kappa-opiate receptor antagonist, nor-binaltorphimine, whereas those of nociceptin were unaffected. Repeated daily administrations of nociceptin resulted in reduced pronociceptive effects, suggestive of the development of tolerance to the hyperalgesic actions of this opioid-related peptide. These findings suggest that the novel peptide, nociceptin, can influence nociceptive responses in the snail, Cepaea, in a manner similar to that indicated for rodents.