Quantitative trait loci for high-temperature adult-plant and slow-rusting resistance to Puccinia striiformis f. sp tritici in wheat cultivars
Quantitative trait loci for high-temperature adult-plant and slow-rusting resistance to Puccinia striiformis f. sp tritici in wheat cultivars
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成株高温和慢锈病抗性条锈菌 f 的数量性状位点。
DOI：
10.1094/phyto-98-7-0803
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发表时间：
2008-07-01
期刊：
影响因子：
3.2
通讯作者：
Zhou, Y.
中科院分区：
文献类型：
--
作者：
Guo, Q.;Zhang, Z. J.;Zhou, Y.
小麦条锈病是小麦（Triticum aestivum）危害最大的病害之一，由小麦条锈病（Puccinia striiformis f. sp. tritici）引起。高温成株抗性（HTAPR）和慢锈病对病害的可持续治理具有很大的潜力。小麦品种卢克（Luke）和阿奎莱贾（Aquileja）以前分别报道具有HTAPR和慢锈性到条锈病。在高温条件下，阿奎莱亚的单位叶面积条纹数比卢克少，卢克的侵染类型比阿奎莱亚低。本研究的目的是确认卢克和阿奎莱贾的抗性并绘制抗性基因图谱。Luke与Aquileja杂交，利用282对微卫星引物对326株F-2植株进行了基因分型。采用高温和低温条件下田间和温室接种的方法，评价了F-2及其衍生的F-3科植物对条锈病的抗性。幼苗和成虫株均记录侵染类型，成虫株仅记录条数。在田间和高温温室中，在2B染色体短臂上发现了两个与成株期侵染类型显著相关的数量性状位点（QTL）。着丝粒远端位点（QYrlu.cau-2BS1）与着丝粒近端位点（QYrlu.cau-2BS2）的遗传距离约为23 cM。QYrlu.cau-2BS1的微卫星标记为Xwmc154和Xgwm148， QYrlu.cau-2BS2的微卫星标记为Xgwm148和Xabrc167。QYrlu.cau-2BS1和QYrlu.cau-2BS2分别解释了36.6%和41.5%的感染型表型变异，总解释率高达78.1%。两个基因座之间没有发现明显的相互作用。在2B染色体长臂上检测到另一个与条纹数显著相关的QTL QYraq.cau-2BL。QYraq.cau-2BL侧有微卫星标记Xwmc175和Xwmc332，可解释条带数表型变异的61.5%。这3个QTL有可能是以前未定位的条锈病抗性位点。
Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most damaging diseases of wheat (Triticum aestivum) globally. High-temperature adult-plant resistance (HTAPR) and slow-rusting have great potential for sustainable management of the disease. The wheat cultivars Luke and Aquileja have been previously reported to possess HTAPR and slow-rusting to stripe rust, respectively. Aquileja displayed less number of stripes per unit leaf area than Luke, while Luke showed lower infection type than Aquileja at adult-plant stages of growth under high-temperature conditions. The objectives of this study were to confirm the resistances and to map the resistance genes in Luke and Aquileja. Luke was crossed with Aquileja, and 326 of the F-2 plants were genotyped using 282 microsatellite primer pairs. These F-2 plants and their derived F-3 families were evaluated for resistance to stripe rust by inoculation in the fields and greenhouses of high- and low-temperatures. Infection type was recorded for both seedlings and adult plants, and stripe number was recorded for adult plants only. Two quantitative trait loci (QTL) were identified, on the short arm of chromosome 2B, to be significantly associated with infection type at adult-plant stages in the fields and in the high-temperature greenhouse. The locus distal to centromere, referred to as QYrlu.cau-2BS1, and the locus proximal to centromere, referred to as QYrlu.cau-2BS2, were separated by a genetic distance of about 23 cM. QYrlu.cau-2BS1 was flanked by the microsatellite markers Xwmc154 and Xgwm148, and QYrlu.cau-2BS2 was flanked by Xgwm148 and Xabrc167. QYrlu.cau-2BS1 and QYrlu.cau-2BS2 explained up to 36.6 and 41.5% of the phenotypic variation of infection type, respectively, and up to 78.1% collectively. No significant interaction between the two loci was detected. Another QTL, referred to as QYraq.cau-2BL, was detected on the long arm of chromosome 2B to be significantly associated with stripe number. QYraq.cau-2BL was flanked by the microsatellite markers Xwmc175 and Xwmc332, and it explained up to 61.5% of the phenotypic variation of stripe number. It is possible that these three QTL are previously un-mapped loci for resistance to stripe rust.