Molecular and cytogenetic characterization of a small alien-segment translocation line carrying the softness genes of Haynaldia villosa.
Molecular and cytogenetic characterization of a small alien-segment translocation line carrying the softness genes of Haynaldia villosa.
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DOI：
10.1139/g2012-051
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发表时间：
2012-08
期刊：
影响因子：
3.1
通讯作者：
Ruiqi Zhang;Xiue Wang;Pei-du Chen
中科院分区：
文献类型：
--
作者：
Ruiqi Zhang;Xiue Wang;Pei-du Chen
小麦-异源小片段易位系（SAST）是小麦改良的重要遗传资源。本研究以簇毛麦（Haynaldia villosa（L.）舒尔普通小麦（Triticum aesitivum L.），全臂小麦H.利用~（60）CO-γ射线辐照毛花易位系T5 VS·5DL，获得了5VS易位系。在BC 2F 2群体中，通过GISH鉴定了6个具有不同5VS片段大小的纯合SAST品系，并使用4个5VS特异性标记和4个基于Ha基因的标记进一步确定了确切的片段大小。结果表明，NAU 5VS-1 ~ NAU 5VS-5共5个品系携带H. NAU 5VS-5具有最小的外源易位片段，为5VS-6AS·6AL末端易位。证明易位染色体5VS-6AS·6AL可稳定遗传。在BC 3F 2代中，5VS-6AS·6AL易位染色体纯合的个体均表现出较软的籽粒质地，其SKCS硬度指数较回交亲本降低约50%。本研究所获得的5VS-6AS·6AL易位系及其分子标记在小麦软粒品质改良中具有重要的应用价值。
The wheat-alien small segment translocation (SAST) lines carrying the beneficial genes from wild species are useful genetic stocks for wheat improvement. In this study, to introduce the grain hardness-related genes of Haynaldia villosa (L.) Schur. into common wheat (Triticum aesitivum L.), the mature female gametes of whole-arm wheat--H. villosa translocation line T5VS·5DL was irradiated by 60CO-γ ray to develop SAST lines involving 5VS. Among the BC2F2 population, six homozygous SAST lines with different fragment sizes of 5VS were identified by GISH, and the exact fragment sizes were further defined using four 5VS-specific markers and four Ha gene-based markers. The results showed that five lines (NAU5VS-1 to NAU5VS-5) carried the softness gene Dina/Dinb of H. villosa, and that NAU5VS-5 had the smallest alien translocation segment, identified to be a 5VS-6AS·6AL terminal translocation. The translocation chromosome 5VS-6AS·6AL was proved to be stably inherited to the successive generations. In the BC3F2 generation, the individuals having the homozygous 5VS-6AS·6AL translocation chromosomes all showed soft grain texture, with an approximately 50% reduction in the SKCS hardness index compared with that of their backcrossing parent. Both the 5VS-6AS·6AL translocation line and the molecular markers developed in this study will be valuable in wheat breeding for soft grain quality improvement.