Partial characterization of the shift from IgG to IgA synthesis in the clonal differentiation of human leukemic bone marrow-derived lymphocytes.
Partial characterization of the shift from IgG to IgA synthesis in the clonal differentiation of human leukemic bone marrow-derived lymphocytes.
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DOI：
10.1084/jem.142.3.549
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发表时间：
1975-09-01
影响因子：
15.3
通讯作者：
Ross, R
中科院分区：
文献类型：
--
作者：
Rudders, R A;Ross, R
在个体（Tun）中观察到不寻常的B细胞增殖，其特征在于在表面和细胞质中存在两个单独的慢性淋巴细胞白血病（CLL）细胞群IgG（k）或伊加（k）染色。利用从相关血清单克隆IgG（k）蛋白制备的独特型抗血清，在IgG和IgA携带细胞群的表面和细胞质中检测独特型。这些观察结果与共同的克隆起源和涉及IgG和伊加合成的开关机制一致。表面IG和细胞内IG与抗血清共轭相反的荧光染料的顺序标记记录的IgA轴承细胞群的终末分化的逐步成熟的水平之前，形态上不同的浆细胞。单个细胞中表面和胞质IgG和伊加染色的分布和模式表明，切换的方向是从IgG合成到伊加合成。IgG和IgA携带人群之间共享独特型特异性的证明与CH基因改变导致的IG重链合成转变一致。它的结论是，某些CLL克隆可能表现出开关从IgG IgA合成的B细胞分化的水平，其中包括B淋巴细胞和Ig合成浆细胞。
An unusual B-cell proliferation was noted in an individual (Tun) which was characterized by the presence of two separate populations of chronic lymphocytic leukemia (CLL) cell staining on the surface and in the cytoplasm for either IgG(k) or IgA(k). Utilizing an idiotypic antiserum prepared from the associated serum monoclonal IgG(k) protein the idiotype was detected on the surface and in the cytoplasm of both the IgG- and IgA-bearing cell populations. These observations are consistent with a common clonal origin and a switch mechanism involving IgG and IgA synthesis. Sequential-labeling of Surface Ig and intracellular Ig with antisera conjugated to opposite fluorochromes documented the progressive maturation of the terminal differentiation of the IgA-bearing cell population at a level before morphologically distinct plasma cells. The distribution and pattern of surface and cytoplasmic IgG and IgA staining in individual cells suggest that the direction of switching is from IgG to IgA synthesis. The demonstration of shared idiotypic specificity between the IgG- and IgA-bearing populations is consistent with a transition in Ig heavy chain synthesis resulting from an alternation in the CH gene. It is concluded that certain CLL clones may manifest a switch from IgG to IgA synthesis at a level of B-cell differentiation which encompasses both the B lymphocyte and the Ig-synthesizing plasma cell.