Hyperosmotic stress response and regulation of cell wall integrity in Saccharomyces cerevisiae share common functional aspects
Hyperosmotic stress response and regulation of cell wall integrity in Saccharomyces cerevisiae share common functional aspects
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DOI：
10.1046/j.1365-2958.2001.02549.x
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发表时间：
2001-08-01
影响因子：
3.6
通讯作者：
Siderius, M
中科院分区：
文献类型：
--
作者：
Alonso-Monge, R;Real, E;Siderius, M
hog1 菌株的渗透敏感表型在高温下受到抑制。在这里，我们表明对于其他常用的 HOG 途径突变株 pbs2 和 sho1ssk2ssk22 来说也是如此，但对于 ste11ssk2ssk22 则不然。相反，ste11ssk2ssk2 菌株在 37 摄氏度下表现出高渗敏感表型。这种表型被 LRE1、HLR1 和 WSC3 的过度表达所抑制，这些基因都已知会影响细胞壁的组成。这些基因对温度诱导的高渗敏感性的抑制促使我们研究 STE11 和其他 HOG 通路成分在细胞完整性中的作用，事实上，我们能够证明 HOG 通路突变体对细胞壁降解酶表现出敏感性。 LRE1 和 HLR1 还被证明可以抑制与 HOG 途径突变体相关的细胞壁表型。此外，分离的多拷贝抑制基因抑制温度诱导的 PKC 途径突变体的细胞裂解表型，这可能表明 PKC 途径和高渗透性反应途径的共享目标。
The osmosensitive phenotype of the hog1 strain is suppressed at elevated temperature. Here, we show that the same holds true for the other commonly used HOG pathway mutant strains pbs2 and sho1ssk2ssk22, but not for ste11ssk2ssk22. Instead, the ste11ssk2ssk2 strain displayed a hyperosmosensitive phenotype at 37 degreesC. This phenotype is suppressed by overexpression of LRE1, HLR1 and WSC3, all genes known to influence cell wall composition. The suppression of the temperature-induced hyperosmosensitivity by these genes prompted us to investigate the role of STE11 and other HOG pathway components in cellular integrity and, indeed, we were able show that HOG pathway mutants display sensitivity to cell wall-degrading enzymes. LRE1 and HLR1 were also shown to suppress the cell wall phenotypes associated with the HOG pathway mutants. In addition, the isolated multicopy suppressor genes suppress temperature-induced cell lysis phenotypes of PKC pathway mutants that could be an indication for shared targets of the PKC pathway and high-osmolarity response routes.