In vitro antiviral activity of the anthraquinone chrysophanic acid against poliovirus
In vitro antiviral activity of the anthraquinone chrysophanic acid against poliovirus
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DOI：
10.1016/s0166-3542(01)00125-5
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发表时间：
2001-03-01
影响因子：
7.6
通讯作者：
Flower, RLP
中科院分区：
文献类型：
--
作者：
Semple, SJ;Pyke, SM;Flower, RLP
大黄酸(1，8-二羟基-3-甲基蒽醌)是从澳大利亚原住民药用植物长叶双胞菌中分离出来的，已被发现在体外抑制脊髓灰质炎病毒2型和3型(小儿麻痹病科)的复制。该化合物对脊髓灰质炎病毒2型和3型的有效浓度分别为0.21和0.02mug/ml，可抑制脊髓灰质炎病毒诱导的BGM(水牛绿猴)肾细胞的细胞病变效应。该化合物抑制了病毒复制周期的早期阶段，但对脊髓灰质炎病毒颗粒没有不可逆转的病毒杀灭作用。大黄酸对其他五种测试的病毒没有显著的抗病毒活性：柯萨奇病毒A21和B4型，人鼻病毒2型(PicorNaviridae)，以及被膜病毒罗斯河病毒(Togaviridae)和单纯疱疹病毒I型(HSV)(疱疹病毒科)。大黄酸、1，8-二羟基大黄酚、大黄素和芦荟大黄素等四种结构上相关的大黄素对脊髓灰质炎病毒3型的抗病毒活性也进行了测试。结果表明，大黄酸分子上的两个疏水位置(C-6和C-3上的甲基)对化合物的抗脊髓灰质炎病毒活性具有重要作用。(C)2001 Elsevier Science B.V.保留所有权利。
Chrysophanic acid (1,8-dihydroxy-3-methylanthraquinone), isolated from the Australian Aboriginal medicinal plant Dianella longifolia, has been found to inhibit the replication of poliovirus types 2 and 3 (Picornaviridae) in vitro. The compound inhibited poliovirus-induced cytopathic effects in BGM (Buffalo green monkey) kidney cells at a 50% effective concentration of 0.21 and 0.02 mug/ml for poliovirus types 2 and 3, respectively. The compound inhibited an early stage in the viral replication cycle, but did not have an irreversible virucidal effect on poliovirus particles. Chrysophanic acid did not have significant antiviral activity against five other viruses tested: Coxsackievirus types A21 and B4, human rhinovirus type 2 (Picornaviridae), and the enveloped viruses Ross River virus (Togaviridae) and herpes simplex virus type 1 (Herpesviridae). Four structurally-related anthraquinones - rhein, 1,8-dihydroxyan-thraquinone, emodin and aloe-emodin were also tested for activity against poliovirus type 3. None of the four compounds was as active as chrysophanic acid against the virus. The results suggested that two hydrophobic positions on the chrysophanic acid molecule (C-6 and the methyl group attached to C-3) were important for the compound's activity against poliovirus. (C) 2001 Elsevier Science B.V. All rights reserved.