Direct manipulation of liquid ordered lipid membrane domains using optical traps
Direct manipulation of liquid ordered lipid membrane domains using optical traps
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DOI：
10.1038/s42004-018-0101-4
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发表时间：
2019-01-29
影响因子：
5.9
通讯作者：
Elani, Yuval
中科院分区：
文献类型：
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作者：
Friddin, Mark S.;Bolognesi, Guido;Elani, Yuval
多组分脂质双分子层可以产生共存的液体结构域，这些结构域被认为影响许多细胞活动。目前还没有直接操纵这些域的方法，阻碍了我们对其重要性的理解。在这里，我们报告了一个系统，允许存在于液体无序矩阵中的单个液体有序域直接使用光镊进行操作。这使我们能够在附着在玻璃表面的巨大囊泡的膜表面上拖动域，从而通过时空控制来定义域的位置。我们还可以使用激光通过混相转变局部加热膜来选择群体中的单个囊泡进行混合/脱混，从而展示进一步的控制层。这项技术有潜力作为一种工具来阐明领域生物物理学，在生物学中的作用，以及在用用户定义的膜图案雕刻膜组件。
Multicomponent lipid bilayers can give rise to coexisting liquid domains that are thought to influence a host of cellular activities. There currently exists no method to directly manipulate such domains, hampering our understanding of their significance. Here we report a system that allows individual liquid ordered domains that exist in a liquid disordered matrix to be directly manipulated using optical tweezers. This allows us to drag domains across the membrane surface of giant vesicles that are adhered to a glass surface, enabling domain location to be defined with spatiotemporal control. We can also use the laser to select individual vesicles in a population to undergo mixing/demixing by locally heating the membrane through the miscibility transition, demonstrating a further layer of control. This technology has potential as a tool to shed light on domain biophysics, on their role in biology, and in sculpting membrane assemblies with user-defined membrane patterning.