Anatomic and Physiologic Heterogeneity of Subgroup-A Auditory Sensory Neurons in Fruit Flies.
Anatomic and Physiologic Heterogeneity of Subgroup-A Auditory Sensory Neurons in Fruit Flies.
复制标题
DOI：
10.3389/fncir.2017.00046
复制
发表时间：
2017
影响因子：
3.5
通讯作者：
Kamikouchi A
中科院分区：
文献类型：
--
作者：
Ishikawa Y;Okamoto N;Nakamura M;Kim H;Kamikouchi A
果蝇的触角耳检测种内通讯中的声学信号，例如求偶歌曲和竞争声音。在蝇耳的五个机械感觉神经元亚群中，A 亚群神经元对 100 至 1,200 Hz 宽频率范围内的振动反应最大。然而，由 A 亚群神经元组成的神经回路的功能组织仍然很大程度上未知。在本研究中，我们使用了11个选择性标记A亚组神经元的GAL4菌株，并通过结合单细胞解剖分析和Ca2+成像来探索A亚组神经元的多样性。我们的研究结果表明，投射到大脑不同分区组合的 A 亚组神经元在解剖学上比之前描述的更加多样化。 A 亚组神经元在生理上也具有多样性，并且某些类型被调谐到较窄的频率范围，这表明 A 亚组神经元对较宽频率范围的声音的反应是由于存在几种类型的 A 亚组神经元。此外，我们发现，当大多数 A 亚组神经元被沉默时，果蝇对求爱歌曲的听觉行为反应就会减弱。总之，这些发现描述了 A 亚组神经元的异质功能组织，这可能有助于物种特异性声信号检测。
The antennal ear of the fruit fly detects acoustic signals in intraspecific communication, such as the courtship song and agonistic sounds. Among the five subgroups of mechanosensory neurons in the fly ear, subgroup-A neurons respond maximally to vibrations over a wide frequency range between 100 and 1,200 Hz. The functional organization of the neural circuit comprised of subgroup-A neurons, however, remains largely unknown. In the present study, we used 11 GAL4 strains that selectively label subgroup-A neurons and explored the diversity of subgroup-A neurons by combining single-cell anatomic analysis and Ca2+ imaging. Our findings indicate that the subgroup-A neurons that project into various combinations of subareas in the brain are more anatomically diverse than previously described. Subgroup-A neurons were also physiologically diverse, and some types were tuned to a narrow frequency range, suggesting that the response of subgroup-A neurons to sounds of a wide frequency range is due to the existence of several types of subgroup-A neurons. Further, we found that an auditory behavioral response to the courtship song of flies was attenuated when most subgroup-A neurons were silenced. Together, these findings characterize the heterogeneous functional organization of subgroup-A neurons, which might facilitate species-specific acoustic signal detection.