Decrease of a peptide in the cat spinal cord after upper cervical transection
Decrease of a peptide in the cat spinal cord after upper cervical transection
复制标题
上颈椎横断术后猫脊髓中肽的减少
DOI：
10.1016/0304-3940(81)90335-9
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发表时间：
1981
影响因子：
2.5
通讯作者：
D. Sutoo
中科院分区：
文献类型：
--
作者：
I. Kanazawa;D. Sutoo
本文研究了猫颈髓横断对脊髓肽含量的影响。在C1水平半切后7天，脊髓的C5-8水平用于生化分析。将粗肽提取物在Sephadex G-15柱上分级分离。收集含有较小分子量肽的第三个主峰，并在纤维素TLC板上用肽图分离。在TLC板上发现了三种茚三酮阳性肽和两种茚三酮阴性肽。当我们在手术侧和对照侧之间进行比较时，在脊髓的手术侧中，茚三酮阴性肽（其酸性水解产物由谷氨酸和天冬氨酸以1：2的比例组成）减少了约30%。
The effects of a transection on the peptide contents in the cat cervical spinal cord have been investigated. Seven days following the hemisection at the C1 level, the C5–8 levels of the spinal cord were used for the biochemical analyses. A crude peptide extract was fractionated on a Sephadex G-15 column. The third major peak which contained smaller molecular weight peptides was collected and separated with a peptide map on the cellulose TLC plate. Three ninhydrin-positive and two ninhydrin-negative peptides were found on the TLC plate. When we compared between the operated side and the control side, a ninhydrin-negative peptide, whose acid hydrolysate was composed of glutamic acid and aspartic acid in a ratio of 1:2, was decreased by approximately 30% in the operated side of the spinal cord.