Analysis of the comparative workflow and performance characteristics of the VITEK 2 and Phoenix systems
Analysis of the comparative workflow and performance characteristics of the VITEK 2 and Phoenix systems
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DOI：
10.1128/jcm.43.8.3829-3834.2005
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发表时间：
2005-08-01
影响因子：
9.4
通讯作者：
Fahr, AM
中科院分区：
文献类型：
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作者：
Eigner, U;Schmid, A;Fahr, AM
VITEK 2（bioMerieux，Marcy L 'Etoile，France）和Phoenix系统（BD诊断系统，Sparks，MD）是用于快速微生物鉴定和敏感性测试的自动化仪器。我们评估了两种仪器的工作流程、获得结果的时间以及鉴定和药敏试验的性能。共检测了307株新鲜临床分离株：141株肠杆菌科，22株非发酵菌，93株葡萄球菌，肠球菌属（Enterococcus spp.）分批测量操作时间，每批7个分离株，共39个批次。对于Phoenix和VITEK 2，每个批次的平均（标准差[SD]）操作时间分别为20.9 +/- 1.8 min和10.6 +/- 1.0 min（P < 0.001）。所有细菌组的平均（+/- SD）结果时间对于Phoenix为727 ± 162 min，对于VITEK 2为506 ± 120 min（P < 0.001）。关于鉴别，Phoenix和VITEK 2对非发酵菌（100%）、葡萄球菌（97%）和肠球菌（100%）产生了相同的结果。对于评价的140株肠杆菌科菌株，Phoenix正确鉴定了135株（96%），VITEK 2正确鉴定了137株（98%）（P = 0.72）。两种仪器所有分离株的总体分类一致率为97.0%。Phoenix和VITEK 2检测的所有细菌分离株的轻微错误率、重大错误率和非常重大错误率分别为3.0、0.3和0.6以及2.8、0.2和1.7（P值分别为0.76、0.75和0.09）。VITEK 2系统所需的手动操作时间和产生结果的时间均少于Phoenix系统。
The VITEK 2 (bioMerieux, Marcy L'Etoile, France) and the Phoenix systems (BD Diagnostic Systems, Sparks, Md.) are automated instruments for rapid organism identification and susceptibility testing. We evaluated the workflow, the time to result, and the performance of identification and susceptibility testing of both instruments. A total of 307 fresh clinical isolates were tested: 141 Enterobacteriaceae, 22 nonfermenters, 93 Staphylococcus spp., and 51 Enterococcus spp. Manipulation time was measured in batches, each with seven isolates, for a total of 39 batches. The mean ( standard deviation [SD]) manipulation time per batch was 20.9 +/- 1.8 min for Phoenix and 10.6 +/- 1.0 min for VITEK 2 (P < 0.001). Mean ( +/- SD) time to result for all bacterial groups was 727 162 min for Phoenix and 506 +/- 120 min for VITEK 2 (P < 0.001). Concerning identification, Phoenix and VITEK 2 yielded the same results for nonfermenters (100%), staphylococci (97%), and enterococci (100%). For 140 Enterobacteriaceae strains evaluated, 135 (96%) were correctly identified by Phoenix and 137 (98%) by VITEK 2 (P = 0.72). The overall category agreement for all isolates was 97.0% for both instruments. The minor error rate, major error rate, and very major error rate for all bacterial isolates tested were 3.0, 0.3, and 0.6 and 2.8, 0.2, and 1.7 for Phoenix and VITEK 2, respectively (P values of 0.76, 0.75, and 0.09). The VITEK 2 system required less manual manipulation time and less time than the Phoenix system to yield results.