Enhancement of human neutrophil adherence by synthetic leukotriene constituents of the slow-reacting substance of anaphylaxis.
Enhancement of human neutrophil adherence by synthetic leukotriene constituents of the slow-reacting substance of anaphylaxis.
复制标题
通过过敏反应的慢反应物质的合成白三烯成分增强人类中性粒细胞的粘附。
作者：
E. Goetzl;L. Brindley;D. Goldman
过敏反应慢反应物质（SRS-A）的主要功能成分，命名为白三烯C4和D4（LTC 4和LTD 4），以及白细胞趋化因子白三烯B4（LTB 4），可增强人中性粒细胞对Sephadex G-25的粘附。当白三烯浓度为3 × 10（-9）M ~ 3 × 10（-7）M时，中性粒细胞粘附的增强是显著的，并且每种白三烯达到最大水平，其幅度与中性粒细胞趋化肽N-甲酰基-甲硫氨酰-亮氨酰苯丙氨酸（FMLP）引起的相似。白三烯和FMLP在37 ℃下1-2分钟内引起中性粒细胞粘附的最佳增加。吲哚美辛抑制了由LTC 4和LTD 4引起的中性粒细胞粘附的增加以及内源性血栓素B2浓度的同时升高。平滑肌收缩和血管活性因子LTC 4和LTD 4缺乏对白细胞的趋化活性，在刺激人中性粒细胞粘附方面与LTB 4一样活跃，并且该作用可能部分由嗜中性粒细胞衍生的血栓素A2介导。
The functionally predominant constituents of the slow-reacting substance of anaphylaxis (SRS-A), designated leukotrienes C4 and D4 (LTC4 and LTD4), as well as the leucocyte chemotactic factor leukotriene B4 (LTB4) enhance the adherence of human neutrophils to Sephadex G-25. Enhancement of neutrophil adherence was significant at leukotriene concentrations of 3 X 10(-9) M -3 X 10(-7) M, and reached a maximum level for each of the leukotrienes that was similar in magnitude to that evoked by the neutrophil chemotactic peptide N-formyl-methionyl-leucylphenylalanine (FMLP). The leukotrienes and FMLP elicited optimum increases in neutrophil adherence within 1-2 min at 37 degrees. Indomethacin inhibited the increase in neutrophil adherence evoked by LTC4 and LTD4 and the concurrent elevation in the concentration of endogenous thromboxane B2. The smooth muscle contractile and vasoactive factors LTC4 and LTD4, which lack chemotactic activity for leucocytes, are as active as LTB4 in stimulating human neutrophil adherence, and the effect may be mediated in part by neutrophil-derived thromboxane A2.