Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood.
Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood.
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DOI：
10.1084/jem.20011756
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发表时间：
2002-03-18
影响因子：
15.3
通讯作者：
Thiel, Andreas
中科院分区：
文献类型：
--
作者：
Kimmig, Sonja;Przybylski, Grzegorz K;Schmidt, Christian A;Laurisch, Katja;Mowes, Beate;Radbruch, Andreas;Thiel, Andreas
在老化的胸腺功能下，无法满足外围T助手（Th）细胞的需求。因此，衰老应导致T细胞受体的损失或稀释，从一个幼稚T细胞的子集中，我们已经确定了人类成人外围血液中的两个幼稚TH细胞的子集，这些血液表征了TREC的不相等，表明与外围CD31相比，TREC高度富集了TREC，与外围CD45RA+ TH缺乏CD31表达的细胞相比，CD31的表达很难。天真的外围TH细胞池在老化但保持幼稚Th细胞的表型和功能特征，因为在体外T细胞受体（TCR）互动上丢失了，我们假设TCR触发是体内稳态驱动的周围驱动的胸腔内胸腔内胸腔内胸腔内及RTES。
During ageing thymic function declines and is unable to meet the demand for peripheral T helper (Th) cell replenishment. Therefore, population maintenance of naive Th cells must be at least partly peripherally based. Such peripheral postthymic expansion of recent thymic emigrants (RTEs) during ageing consequently should lead to loss or dilution of T cell receptor excision circles (TRECs) from a subset of naive T cells. We have identified two subsets of naive Th cells in human adult peripheral blood characterized by a striking unequal content of TRECs, indicating different peripheral proliferative histories. TRECs are highly enriched in peripheral naive CD45RA+ Th cells coexpressing CD31 compared with peripheral naive CD45RA+ Th cells lacking CD31 expression, in which TRECs can hardly be detected. Furthermore we show that CD31−CD45RA+ Th cells account for increasing percentages of the naive peripheral Th cell pool during ageing but retain phenotypic and functional features of naive Th cells. As CD31 is lost upon T cell receptor (TCR) engagement in vitro, we hypothesize that TCR triggering is a prerequisite for homeostatically driven peripheral postthymic expansion of human naive RTEs. We describe here the identification of peripherally expanded naive Th cells in human adult blood characterized by the loss of CD31 expression and a highly reduced TREC content.