The Effects of Cholesterol Oxidation on Erythrocyte Plasma Membranes: A Monolayer Study.
The Effects of Cholesterol Oxidation on Erythrocyte Plasma Membranes: A Monolayer Study.
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DOI：
10.3390/membranes12090828
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发表时间：
2022-08-24
期刊：
影响因子：
4.2
通讯作者：
Petrov PG
中科院分区：
文献类型：
--
作者：
Lechner BD;Smith P;McGill B;Marshall S;Trick JL;Chumakov AP;Winlove CP;Konovalov OV;Lorenz CD;Petrov PG
胆固醇在脂质膜的分子和介观组织中起关键作用，并且预期其分子结构的变化（例如，通过环境因素如氧化应激）可能不利地影响膜的性质和功能。在这项研究中，我们提出的证据表明，胆固醇氧化的机械性能，分子和介观组织和脂质-甾醇的相互作用在凝聚的单层组成的主要物种中发现的内小叶的红细胞膜有显着的影响。综合运用各种实验方法（静态面积压缩性，表面流变学，荧光显微镜和表面敏感的X射线技术）和原子分子动力学模拟，我们表明，胆固醇氧化为7-酮胆固醇导致单分子层硬化（在静态和动态条件下），单层微区组织的显著变化，货车范德华力的破坏，甾醇和其他脂质物质之间的静电和疏水相互作用，以及脂质膜水合作用。表面敏感的X-射线技术表明，虽然在这些凝聚相的胆固醇氧化的分子包装模式没有显着影响，有微妙的变化，膜厚度和含7-酮胆固醇的单层的相干长度显着减少。
Cholesterol plays a key role in the molecular and mesoscopic organisation of lipid membranes and it is expected that changes in its molecular structure (e.g., through environmental factors such as oxidative stress) may affect adversely membrane properties and function. In this study, we present evidence that oxidation of cholesterol has significant effects on the mechanical properties, molecular and mesoscopic organisation and lipid–sterol interactions in condensed monolayers composed of the main species found in the inner leaflet of the erythrocyte membrane. Using a combination of experimental methods (static area compressibility, surface dilatational rheology, fluorescence microscopy, and surface sensitive X-ray techniques) and atomistic molecular dynamics simulations, we show that oxidation of cholesterol to 7-ketocholesterol leads to stiffening of the monolayer (under both static and dynamic conditions), significant changes in the monolayer microdomain organisation, disruption in the van der Waals, electrostatic and hydrophobic interactions between the sterol and the other lipid species, and the lipid membrane hydration. Surface sensitive X-ray techniques reveal that, whilst the molecular packing mode is not significantly affected by cholesterol oxidation in these condensed phases, there are subtle changes in membrane thickness and a significant decrease in the coherence length in monolayers containing 7-ketocholesterol.
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影响因子：
3.3
作者：
Klauda, Jeffery B.;Venable, Richard M.;Freites, J. Alfredo;O'Connor, Joseph W.;Tobias, Douglas J.;Mondragon-Ramirez, Carlos;Vorobyov, Igor;MacKerell, Alexander D., Jr.;Pastor, Richard W.
通讯作者：
Pastor, Richard W.
影响因子：
3.3
作者：
Bera, Indrani;Klauda, Jeffery B.
通讯作者：
Klauda, Jeffery B.
影响因子：
15.6
作者：
Brezesinski, G;Möhwald, H
通讯作者：
Möhwald, H
影响因子：
4.8
作者：
Bokori-Brown, Monika;Petrov, Peter G.;Winlove, C. Peter
通讯作者：
Winlove, C. Peter
DOI：
10.1051/jp2:1991102
发表时间：
1991-02-01
期刊：
JOURNAL DE PHYSIQUE II
影响因子：
--
作者：
DAILLANT, J;BOSIO, L;BENATTAR, JJ
通讯作者：
BENATTAR, JJ