New insights into tumor microstructure using temporal diffusion spectroscopy.
New insights into tumor microstructure using temporal diffusion spectroscopy.
复制标题
使用时间扩散光谱法对肿瘤微结构进行新的见解。
DOI：
10.1158/0008-5472.can-08-0832
复制
发表时间：
2008-07-15
期刊：
影响因子：
11.2
通讯作者：
Gore JC
中科院分区：
文献类型：
--
作者：
Colvin DC;Yankeelov TE;Does MD;Yue Z;Quarles C;Gore JC
描绘组织中水扩散速率的磁共振图像 (MRI) 可用于表征肿瘤的细胞结构，并且对于评估肿瘤对治疗的早期反应很有价值。水扩散速率对组织的细胞和分子含量敏感，并受到与肿瘤发展相关的局部微观结构变化的影响。然而，传统的水扩散图反映了在多个尺度上对自由扩散的限制的综合影响，直至特定的限制空间维度（通常为几微米）。这种测量无法区分较小规模的结构变化引起的影响。扩散速率的变化很大程度上反映了细胞密度的变化，并且没有关于亚细胞尺度变化的信息。我们在此报告我们使用基于振荡梯度自旋回波 (OGSE) MRI 方法的新方法的经验，该方法可以区分比单细胞直径小得多的尺度上的结构变化对水扩散的影响。当扩散测量选择性地对较短距离尺度敏感时，体内大鼠脑胶质母细胞瘤的 MRI 显示对比度和空间异质性增加。这些结果显示了 OGSE 方法在揭示体内肿瘤微观变化方面的优势，并证实扩散测量取决于细胞结构以外的因素。
Magnetic resonance images (MRI) that depict rates of water diffusion in tissues can be used to characterize the cellularity of tumors and are valuable in assessing their early response to treatment. Water diffusion rates are sensitive to the cellular and molecular content of tissues and are affected by local microstructural changes associated with tumor development. However, conventional maps of water diffusion reflect the integrated effects of restrictions to free diffusion at multiple scales up to a specific limiting spatial dimension, typically several micrometers. Such measurements cannot distinguish effects caused by structural variations at a smaller scale. Variations in diffusion rates then largely reflect variations in the density of cells, and no information is available about changes on a subcellular scale. We report here our experiences using a new approach based on Oscillating Gradient Spin-Echo (OGSE) MRI methods that can differentiate the influence on water diffusion of structural changes on scales much smaller than the diameter of a single cell. MRIs of glioblastomas in rat brain in vivo show an increased contrast and spatial heterogeneity when diffusion measurements are selectively sensitized to shorter distance scales. These results show the benefit of OGSE methods for revealing microscopic variations in tumors in vivo and confirm that diffusion measurements depend on factors other than cellularity.