Sialyl residues in hepatitis B antigen: their role in determining the life span of the antigen in serum and in eliciting an immunological response.
Sialyl residues in hepatitis B antigen: their role in determining the life span of the antigen in serum and in eliciting an immunological response.
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乙型肝炎抗原中的唾液酸残基：它们在确定血清中抗原的寿命和引发免疫反应中的作用。
DOI：
10.1099/0022-1317-27-1-81
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
A. Prince
中科院分区：
文献类型：
--
作者：
A. Neurath;N. Hashimoto;A. Prince
B型肝炎表面抗原被吸附到从鲎血淋巴中分离的不溶性唾液酸特异性血凝素上。用霍乱弧菌神经氨酸酶（EC 3.2.1.18）处理抗原导致唾液酸的释放和等电点从pH 4-35（对于亚型ad）或4-9（对于亚型ay）增加到pH 5-45。当在37 ℃下与唾液酸转移酶（EC 2.4.99.1）和胞苷-5 '-单磷酸-[14-C]-唾液酸孵育时，经处理但未经处理的抗原掺入[14-C]-唾液酸。[14-C]-唾液酸的主要部分与一个表观摩尔比为1的糖蛋白连接。重量26 x 10-a。去唾液酸化抗原在兔血浆中的体内寿命大大缩短，并且比完整抗原（亚型ad）引起更高的体液抗体应答。免疫后3个月测量的抗原刺激的淋巴细胞增殖，只观察到与神经氨酸酶处理的抗原注射的兔细胞。
Hepatitis B surface antigen was adsorbed to insolubilized sialic acid-specific haemagglutinin isolated from the haemolymph of Limulus polyphemus. Treatment of the antigen with Vibrio cholerae neuraminidase (EC 3.2.1.18) resulted in the release of sialic acid and in an increase of the isoelectric point from pH 4-35 (for subtype ad) or 4-9 (for subtype ay) to pH 5-45. Treated, but not untreated, antigen incorporated [14-C]-sialic acid when incubated at 37 degrees C with sialyl transferase (EC 2.4.99.1) and cytidine-5'-monophosphate-[14-C]-sialic acid. The major portion of [14-C]-sialic acid was linked to a glycoprotein with an apparent mol. wt. of 26 x 10-a. De-sialylated antigen had a drastically reduced in vivo life span in rabbit plasma and elicited a higher humoral antibody response than intact antigen (subtype ad). Antigen-stimulated proliferation of lymphocytes, measured 3 months after immunization, was observed only with cells from rabbits injected with neuraminidase-treated antigen.