Establishment and characterization of a porcine kidney cell line, FS-L3, which forms unique multicellular domes in serum-free culture
Establishment and characterization of a porcine kidney cell line, FS-L3, which forms unique multicellular domes in serum-free culture
复制标题
猪肾细胞系 FS-L3 的建立和表征，该细胞系在无血清培养中形成独特的多细胞圆顶
DOI：
10.1007/s11626-998-0053-6
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
A. Fukusho
中科院分区：
文献类型：
--
作者：
Y. Sakoda;A. Fukusho
建立了稳定的猪肾上皮细胞系FS-L3，并在Eagle‘s最低基本培养液中建立和维持了该细胞系，该细胞系含有0.295%的胰糖磷酸肉汤、0.5%的BACTO蛋白胨和10 mM的N，N-双(2-羟乙基)-2-氨基乙磺酸。染色体的模式是37到38。FS-L3细胞在单层上形成充满流体的、多细胞的三维穹顶。经过进一步培养后，穹顶的数量明显增加。通过使用Pre-1进行杂交，该细胞系的起源被确认为猪，该基因可以作为猪基因组中的特定序列进行检测。还发现FS-L3细胞不存在可能的外源病毒和支原体。
SummaryA stable porcine kidney epithelial cell line, FS-L3, was established and maintained in Eagle’s minimum essential medium containing 0.295% tryptose phosphate broth, 0.5% Bacto Peptone, and 10 mM N, N-Bis (2-hydroxyethyl)-2-aminoethanesulfonic acid without any serum. The mode of chromosomes is 37 to 38. The FS-L3 cells formed fluid-filled, multicellular, three-dimensional domes on a single monolayer. The number of domes increased markedly after further cultivation. The origin of this cell line was confirmed as porcine by hybridization using PRE-1, which can be detected as a specific sequence in the porcine genome. It was also found that FS-L3 cells were free from possible adventitious viruses and mycoplasmas.
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
--