Studies of the rosette inhibition test in pregnant mice: evidence of immunosuppression?
Studies of the rosette inhibition test in pregnant mice: evidence of immunosuppression?
复制标题
怀孕小鼠玫瑰花结抑制试验的研究：免疫抑制的证据？
DOI：
10.1098/rspb.1976.0054
复制
发表时间：
1976
期刊：
影响因子：
--
通讯作者：
G. Clunie
中科院分区：
文献类型：
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作者：
H. Morton;V. Hegh;G. Clunie
玫瑰花结抑制试验已被用作器官移植后免疫抑制的衡量标准，我们最近证明它也可用于检测怀孕小鼠中淋巴细胞反应性的抑制状态。如果淋巴细胞反应性的降低提供了免疫抑制的迹象，那么这项研究表明，在小鼠中，脾脏淋巴细胞早在受精后 4 小时就受到免疫抑制。这种状态持续 13-15 天，在足月前 4-6 天恢复正常。通过将这些淋巴细胞与交配后 6 小时和 24 小时取自怀孕小鼠的血清一起孵育，可以在体外诱导正常脾淋巴细胞的类似抑制。检测到的血清因子在 56 °C 加热时稳定，但在 72 °C 加热时不稳定，并且不可透析。它在稀释度为 64 分之一的血清中被检测到。正在开展进一步的工作来研究这一现象并追求妊娠因子的纯化和鉴定。
The rosette inhibition test has been utilized as a measure of immunosuppression following organ transplantation, and we have recently demonstrated that it can also be used in detecting a state of depression of lymphocyte reactivity occurring in pregnant mice. If the depression of lymphocyte reactivity provides an indication of immunosuppression, then this study has shown that, in the mouse, the spleen lymphocytes are immunosuppressed as early as 4 h after fertilization. This state persists for 13–15 days, with a return to normal 4–6 days before full term. A similar depression of normal spleen lymphocytes can be induced in vitro by incubating these lymphocytes with serum taken from pregnant mice 6 and 24 h after mating. The serum factor detected was shown to be stable when heated at 56 °C but not at 72 °C, and to be non-dialysable. It was detected in serum to a dilution of 1 in 64. Further work is being undertaken to investigate this phenomenon and to pursue the purification and identification of the pregnancy factor.