Nitrated and oxidized plasma proteins in smokers and lung cancer patients.
Nitrated and oxidized plasma proteins in smokers and lung cancer patients.
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发表时间：
2001
期刊：
影响因子：
11.2
通讯作者：
B. Pignatelli;Chun-Qi Li;P. Boffetta;Qiping Chen;W. Ahrens;F. Nyberg;A. Mukeria;I. Brüske-Hohlfeld;C. Fortes;V. Constantinescu;H. Ischiropoulos;H. Ohshima
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作者：
B. Pignatelli;Chun-Qi Li;P. Boffetta;Qiping Chen;W. Ahrens;F. Nyberg;A. Mukeria;I. Brüske-Hohlfeld;C. Fortes;V. Constantinescu;H. Ischiropoulos;H. Ohshima
吸烟是导致肺癌和其他呼吸系统疾病的原因之一。存在于香烟烟雾和/或在吸烟者肺部形成的氧化剂可能引发DNA和细胞成分的氧化和硝化损伤，从而导致致癌。我们使用免疫点和免疫印迹分析来测量52名肺癌患者和43名对照受试者（重度和轻度吸烟者，有或没有接触环境烟草烟雾的不吸烟者）血浆样本中的硝化（含硝基酪氨酸）和氧化（含羰基）蛋白。肺癌患者的硝化蛋白水平明显高于对照组（P = 0.003）。另一方面，吸烟者的氧化蛋白水平明显高于不吸烟者（P < 0.001）。Western-blot分析显示存在2 - 5种硝化蛋白和1种氧化蛋白。使用免疫沉淀和Western-blot分析8种不同的人血浆蛋白抗体，我们鉴定出纤维蛋白原、转铁蛋白、纤溶酶原和铜蓝蛋白是硝化蛋白，纤维蛋白原是肺癌患者和吸烟者血浆中唯一的氧化蛋白。我们的研究结果表明，吸烟增加了氧化应激，在肺癌的发展过程中，活性氮的形成导致血浆蛋白的硝化和氧化。
Cigarette smoking is a cause of lung cancer and other respiratory diseases. Oxidants either present in cigarette smoke and/or formed in the lung of smokers may trigger oxidative and nitrative damage to DNA and cellular components, contributing to carcinogenesis. We have used immunodot and Western blot analyses to measure nitrated (nitrotyrosine-containing) and oxidized (carbonyl-containing) proteins in plasma samples collected from 52 lung cancer patients and 43 control subjects (heavy and light smokers, nonsmokers with or without exposure to environmental tobacco smoke). The levels of nitrated proteins were significantly higher in lung cancer patients than in controls (P = 0.003). On the other hand, the levels of oxidized proteins were significantly higher in smokers than in nonsmokers (P < 0.001). Western-blot analyses showed the presence of two to five nitrated proteins and one oxidized protein. Using immunoprecipitation and Western-blot analyses with eight different antibodies against human plasma proteins, we identified fibrinogen, transferrin, plasminogen, and ceruloplasmin as nitrated proteins and fibrinogen as the only oxidized protein present in human plasma of lung cancer patients and smokers. Our results indicate that cigarette smoking increases oxidative stress and that during lung cancer development, formation of reactive nitrogen species results in nitration and oxidation of plasma proteins.