Potential of Human Saliva for Nuclear Magnetic Resonance-Based Metabolomics and for Health-Related Biomarker Identification
Potential of Human Saliva for Nuclear Magnetic Resonance-Based Metabolomics and for Health-Related Biomarker Identification
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DOI：
10.1021/ac9020598
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发表时间：
2009-11-01
影响因子：
7.4
通讯作者：
Eller, Nanna
中科院分区：
文献类型：
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作者：
Bertram, Hanne Christine;Eggers, Nina;Eller, Nanna
在本研究中，（1）H核磁共振（NMR）的能力，人类唾液样本的代谢谱进行了研究。获得高分辨率（1）H NMR谱，并将信号分配给主要代表小有机酸和氨基酸的各种代谢产物。此外，使用人唾液代谢组学研究进行了评估，多变量数据分析显示，92个早晨和晚上的样本从46个科目可以区分与85%的预测性。对唾液代谢物谱的昼夜效应归因于几种代谢物强度的变化，包括氧化三甲胺（TMAO）、胆碱、丙酸盐、丙氨酸、甲醇和N-乙酰基。未检测到性别和体重指数（BMI）对唾液代谢产物谱的影响。研究了唾液代谢组与糖化血红蛋白、收缩压和舒张压之间的关系;然而，未建立显著相关性。
In the present study, the ability of (1)H nuclear magnetic resonance (NMR) for metabolic profiling of human saliva samples was investigated. High-resolution (1)H NMR spectra were obtained, and signals were assigned to various metabolites mainly representing small organic acids and amino acids. In addition, the use of human saliva for metabolomic studies was evaluated, and multivariate data analysis revealed that the 92 morning and night samples from 46 subjects could be discriminated with a predictability of 85%. The diurnal effect on the salivary metabolite profile were ascribed to changes in intensities of several metabolites including trimethylamine oxide (TMAO), choline, propionate, alanine, methanol, and N-acetyl groups. No effects of gender and body mass index (BMI) on the salivary metabolite profile were detected. The relationships between the salivary metabolome and glycated hemoglobin, systolic and diastolic blood pressure were investigated; however, no significant correlations could be established.