Oscillatory deformation of human erythrocytes in sinusoidally modulated shear flow.
Oscillatory deformation of human erythrocytes in sinusoidally modulated shear flow.
复制标题
正弦调制剪切流中人红细胞的振荡变形。
DOI：
10.3233/bir-1988-251-211
复制
发表时间：
1988
期刊：
影响因子：
1.1
通讯作者：
T. Shiga
中科院分区：
文献类型：
--
作者：
K. Kon;J. Murakami;K. Takaoka;T. Shiga
研究了红细胞在振荡剪切力作用下的变形。剪切应力在8和32达因/厘米2之间正弦调制，因此，细胞变形的程度正弦改变。在较低的调制频率（小于1.8 Hz）下，完整的红细胞对施加在细胞上的剪应力有完美的反应，并且它们的变形程度与稳态剪切流中的变形程度一样大。高于2 Hz时，胞状变形不能跟随剪切应力的变化沿着向上相位的剪切应力循环。随着细胞内血红蛋白浓度的降低，细胞对振荡剪切力的反应性变好。用低浓度的二酰胺处理细胞损害了完整细胞对振荡剪切应力的反应，但不影响部分溶血细胞的反应。这些数据表明，细胞对振荡剪切应力的反应是由细胞骨架结构和细胞内粘度决定的。
The red cell deformation under oscillatory shear stress was studied. Shear stress was sinusoidally modulated between 8 and 32 dyn/cm2, thus, the extent of cellular deformation altered sinusoidally. At a low modulation frequency (less than 1.8 Hz), intact red cells perfectly responded to the shear stress applied on cells, and they could deform as much as the deformation in stationary shear flow. Above 2 Hz, the cellular deformation could not follow changes in shear stress along up-phase in the shear stress cycle. As decreasing the intracellular hemoglobin concentration, the cellular response to oscillatory shear stress became better. Treatment of cells with low concentrations of diamide impaired the response of intact cells to oscillatory shear stress, but unaffected the response of partially hemolyzed cells. These data suggest that the cellular response to oscillatory shear stress is determined by the cytoskeletal structure and the intracellular viscosity.