2 SMALL RNAS ENCODED BY EPSTEIN-BARR VIRUS AND COMPLEXED WITH PROTEIN ARE PRECIPITATED BY ANTIBODIES FROM PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS
2 SMALL RNAS ENCODED BY EPSTEIN-BARR VIRUS AND COMPLEXED WITH PROTEIN ARE PRECIPITATED BY ANTIBODIES FROM PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS
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DOI：
10.1073/pnas.78.2.805
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
STEITZ, JA
中科院分区：
文献类型：
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作者：
LERNER, MR;ANDREWS, NC;STEITZ, JA
携带爱泼斯坦 - 巴尔病毒（EBV）基因组的灵长类细胞会合成大量的两种小RNA：EBER 1和EBER 2（EBV编码的RNA）。这些RNA长约180个核苷酸，具有5′ pppA末端，且无多聚（A）尾。它们具有不同的T1和胰核糖核酸酶消化指纹图谱。在正常的B淋巴细胞、缺乏EBV DNA的转化B淋巴细胞、被蛛猴疱疹病毒转化的T淋巴细胞或多种其他非淋巴细胞的哺乳动物细胞中均未发现它们。杂交分析表明，EBER 1和EBER 2由EBV（B95 - 8）DNA的EcoRI - J片段编码。在体内，这两种RNA都与蛋白质结合，从而可被系统性红斑狼疮相关抗体抗 - La特异性沉淀。未感染的哺乳动物细胞中的La抗原由一类异质性的小核糖核蛋白颗粒组成，其中一些RNA成分与一种高度重复、散布的人类DNA类别（称为Alu家族）表现出序列同源性。文中讨论了EBER 1和EBER 2在EBV感染和细胞转化中的可能功能，以及它们与系统性红斑狼疮发病机制的潜在关系。
Primate cells harboring the Epstein-Barr virus (EBV) genome synthesize large amounts of 2 small RNA species: EBER 1 and EBER 2 (EBV-encoded RNA). These RNA are .apprx. 180 nucleotides long, possess 5'' pppA termini, and lack poly(A). They have different T1 and pancreatic RNase digestion fingerprints. They are not found in normal B lymphocytes, in transformed B lymphocytes that lack EBV DNA, in T lymphocytes transformed by Herpesvirus ateles, or in a variety of other nonlymphoid mammalian cells. Hybridization analyses indicate that EBER 1 and EBER 2 are encoded by the EcoRI-J fragment of EBV (B95-8) DNA. In vivo both RNA are associated with protein(s), allowing their specific precipitation by the systemic lupus erythematosus-associated antibody anti-La. The La antigen in uninfected mammalian cells consists of a heterogeneous class of small ribonucleoprotein particles, some of whose RNA components exhibit sequence homology with a highly repetitive, interspersed class of human DNA designated the Alu family. Possible functions for EBER 1 and EBER 2 in infection and cell transformation by EBV and their potential relationship to the pathogenesis of systemic lupus erythematosus are discussed.