Analysis of the molecular species of the chick oviduct progesterone receptor using isoelectric focusing.
Analysis of the molecular species of the chick oviduct progesterone receptor using isoelectric focusing.
复制标题
使用等电聚焦分析鸡输卵管孕酮受体的分子种类。
DOI：
10.1021/bi00584a007
复制
发表时间：
1979
期刊：
影响因子：
2.9
通讯作者：
T. Spelsberg
中科院分区：
文献类型：
--
作者：
P. Boyd;T. Spelsberg
描述了在来自小鸡输卵管的黄体酮受体的Sephadex平床上进行制备性等电聚焦的条件。该方法可以将受体分离成两种分子种类，一种集中在 pI 6，另一种集中在 pI 7，并具有良好的纯化和回收率。 pI 6 和pI 7 受体种类分别纯化2 倍和26 倍。使用木炭结合法对聚焦组分进行分析可准确识别和定量[3H]孕酮受体。该方法在两种受体种类的回收、定量和分辨率方面具有可重复性。在线性蔗糖梯度上，表观 pI 为 6 的沉积物在大约 4 S 处，而受体在大约 3.5 S 处的表观 pI 沉积物为 7。根据二乙氨基乙基 (DEAE) 色谱的沉降值和洗脱模式，pI 6 组分相当于“B”受体种类，pI 7 组分相当于先前描述的“A”受体种类 [schrader, W, T., 7 O'Malley, B.W.)1972) J.Biol。化学。 241, 51--59]。
Conditions are described for the preparative isoelectric focusing in flat beds of Sephadex of the progesterone receptor from the chick oviduct. The method allows the fractionation of the receptor into two molecular species, one focusing at pI 6 and the other at pI 7 with good purification and recovery. The pI 6 and pI 7 receptor species were purified 2- and 26-fold, respectively. The assaying of the focused fractions with the charcoal binding method provides an accurate identification and quantitation of the [3H]progesterone receptor. The method is reproducible in recovery, quantitation, and resolution of the two receptor species. The receptor with an apparent pI of 6 sediments at approximately 4 S on linear sucrose gradients, while the receptor with an apparent pI of 7 sediments at approximately 3.5 S. On the basis of the sedimentation values and elution patterns from diethylaminoethyl (DEAE) chromatography, the pI 6 component is equivalent to the "B" receptor species and the pI 7 component is equivalent to the "A" receptor species described previously [schrader, W, T., 7 O'Malley, B. W. )1972) J. Biol. Chem. 241, 51--59].