High Metabolic Potential May Contribute to the Success of ST131 Uropathogenic Escherichia coli
High Metabolic Potential May Contribute to the Success of ST131 Uropathogenic Escherichia coli
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DOI：
10.1128/jcm.01423-12
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发表时间：
2012-10-01
影响因子：
9.4
通讯作者：
Upton, Mathew
中科院分区：
文献类型：
--
作者：
Gibreel, Tarek M.;Dodgson, Andrew R.;Upton, Mathew
尿路致病性大肠杆菌（UPEC）是医院和社区尿路感染的主要原因。最近出现的多药耐药克隆，如O25 b：H4-ST 131谱系，对健康构成了重大威胁，许多研究已经探索了这些生物体的毒力潜力。ST 131克隆的成员被描述为具有可变的关键毒力因子携带，并且已经表明其他未鉴定的因子有助于毒力。在这里，我们证明了ST 131分离株具有高代谢潜力和生化特征，将其与许多其他序列类型（ST）的分离株区分开来。收集的300 UPEC分离株回收在2007年和2009年在英格兰西北地区进行代谢分析，使用Vitek 2高级专家系统（AES）。在进行的47次检测中，30次检测结果为阳性，300个分离株中至少有一个得到了检测结果。ST 131菌株表现出显着的关联与八个测试，包括肽酶，脱羧酶，碱化活性。代谢活性也与抗生素敏感性相关，耐药微生物显示出最高的代谢潜力。这是第一次全面研究ST 131谱系的代谢潜力，我们认为高代谢潜力可能有助于ST 131克隆成员的适应性，这些成员能够利用肠道和泌尿道环境中的可用营养物质。
Uropathogenic Escherichia coli (UPEC) is the predominant cause of urinary tract infection in both hospital and community settings. The recent emergence of multidrug-resistant clones like the O25b:H4-ST131 lineage represents a significant threat to health, and numerous studies have explored the virulence potential of these organisms. Members of the ST131 clone have been described as having variable carriage of key virulence factors, and it has been suggested that additional unidentified factors contribute to virulence. Here we demonstrated that ST131 isolates have high metabolic potential and biochemical profiles that distinguish them from isolates of many other sequence types (STs). A collection of 300 UPEC isolates recovered in 2007 and 2009 in the Northwest region of England were subjected to metabolic profiling using the Vitek2 Advanced Expert System (AES). Of the 47 tests carried out, 30 gave a positive result with at least one of the 300 isolates examined. ST131 isolates demonstrated significant association with eight tests, including those for peptidase, decarboxylase, and alkalinization activity. Metabolic activity also correlated with antibiotic susceptibility profiles, with resistant organisms displaying the highest metabolic potential. This is the first comprehensive study of metabolic potential in the ST131 lineage, and we suggest that high metabolic potential may have contributed to the fitness of members of the ST131 clone, which are able to exploit the available nutrients in both the intestinal and urinary tract environments.