Blastoderm-differential and blastoderm-specific genes of Drosophila melanogaster.
Blastoderm-differential and blastoderm-specific genes of Drosophila melanogaster.
复制标题
果蝇的胚盘分化和胚盘特异性基因。
DOI：
10.1016/0012-1606(85)90473-7
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发表时间：
1985
影响因子：
2.7
通讯作者：
Lengyel,JA
中科院分区：
文献类型：
--
作者：
Roark,M;Mahoney,PA;Graham,ML;Lengyel,JA
我们通过分子克隆的方法分离了果蝇胚胎期差异表达的基因。其中两个胚层差异基因在后期重新表达，并定位到单个染色体位点95C和99E。99E处的序列是编码肌球蛋白轻链2的序列。另外两个胚层差异序列是多基因家族的成员(其中一个是B104，或roo)，并映射到多个分散的染色体位点。发现了一个原肠胚差异序列，该序列映射到71a。最重要的是，我们已经确定了三个编码在胚层阶段唯一表达的转录本的基因；这些基因映射到单个染色体位点：25D3、75C和99D4-8。至少有一些胚层分化和胚层特异的基因座似乎与胚胎模式形成有关的基因座不同，这些基因座已在最近的遗传“饱和”筛查中被鉴定出来。因此，识别胚芽阶段特有基因的程序可能允许识别与重要胚胎过程有关的基因，这些基因以前没有被经典的基因技术识别出来。
We have isolated, by molecular cloning, genes expressed differentially at the blastoderm stage ofDrosophila melanogaster. Two of the blastoderm-differential genes are reexpressed at later stages, and map to single chromosomal loci 95C and 99E. The sequence at 99E is that encoding the myosin light chain 2. Two other blastoderm-differential sequences are members of multigene families (one of which is B104, or roo) and map to multiple dispersed chromosomal loci. A gastrula-differential sequence was found which maps to 71A. Most significantly, we have identified three genes encoding transcripts expressed uniquely at the blastoderm stage; these map to single chromosomal loci: 25D3, 75C, and 99D4-8. At least some of the blastoderm-differential and blastoderm-specific loci appear to be distinct from loci involved in embryonic pattern formation that have been identified in recent genetic “saturation” screens. The procedure of identifying genes specific to the blastoderm stage may thus allow the identification of genes, not previously identified by classical genetic techniques, that are involved in important embryonic processes.