V2 of tomato yellow leaf curl virus can suppress methylation-mediated transcriptional gene silencing in plants
V2 of tomato yellow leaf curl virus can suppress methylation-mediated transcriptional gene silencing in plants
复制标题
番茄黄曲叶病毒V2可抑制植物中甲基化介导的转录基因沉默
DOI：
10.1099/vir.0.055798-0
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发表时间：
2014
影响因子：
3.8
通讯作者：
Zhou Xueping
中科院分区：
文献类型：
--
作者：
Wang Bi;Li Fangfang;Huang Changjun;Yang Xiuling;Qian Yajuan;Xie Yan;Zhou Xueping
番茄黄化曲叶病毒（Tomato yellow leaf curl virus，TYLCV）是菜豆病毒属的一种DNA病毒。TYLCV利用双链DNA中间体进行复制，该中间体可以成为植物转录基因沉默（TGS）的靶标。在本研究中，我们发现TYLCV的V2蛋白可以抑制烟草本氏菌素16-TGS中转录沉默的绿色荧光蛋白（GFP）转基因的TGS。通过亚硫酸氢盐测序和chop-PCR，我们证明TYLCV V2可以通过降低35 S启动子序列中的甲基化水平来逆转GFP转基因沉默。拟南芥中的AtSN 1和MEA-ISR基因座都被强烈甲基化，我们发现这两个基因座的甲基化状态在TYLCV V2转基因拟南芥植株中显著降低。因此，TYLCV在侵染植物时能有效抑制TGS，其V2蛋白是TGS抑制活性的主要来源。
Tomato yellow leaf curl virus(TYLCV) is a DNA virus belonging to the genusBegomovirus. TYLCV replicates using double-stranded DNA intermediates that can become the target of plant transcriptional gene silencing (TGS). Here, we show that the V2 protein of TYLCV can suppress TGS of a transcriptionally silenced green fluorescent protein (GFP) transgene inNicotiana benthamianaline 16-TGS. Through bisulfite sequencing and chop-PCR, we demonstrated that the TYLCV V2 can reverse GFP transgene silencing by reducing the methylation levels in the 35S promoter sequence. BothAtSN1andMEA-ISRloci inArabidopsis thalianawere previously reported to be strongly methylated, and we show that the methylation status of both loci was significantly reduced in TYLCV V2 transgenicArabidopsisplants. We conclude that TYLCV can efficiently suppress TGS when it infects plants, and its V2 protein is responsible for the TGS suppression activity.