Identification and immunohistochemical characterization of colospinal afferent neurons in the rat
Identification and immunohistochemical characterization of colospinal afferent neurons in the rat
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DOI：
10.1016/j.neuroscience.2008.02.046
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发表时间：
2008-05-15
期刊：
影响因子：
3.3
通讯作者：
Caudle, R. M.
中科院分区：
文献类型：
--
作者：
Suckow, S. K.;Caudle, R. M.
小肠和大肠中肠神经元的分类、形态和功能已被广泛研究。然而，对直接投射到CNS的肠神经元知之甚少。以前的研究已经确定了这些独特的神经元在直肠，直肠脊髓神经元，但很少做表征他们。因此，本研究的目的是通过逆行神经元追踪和免疫组织化学方法鉴定和表征大鼠结肠中直接投射到CNS的肠神经元。应用1，1 ′-双十八烷基-3，3，3 ′，3 ′-四甲基吲哚羰花青高氯酸盐（Dil）和荧光金（FG）逆行示踪脊髓L6/S1节段，我们在结肠肌间神经丛和粘膜下神经丛中鉴定了这些神经元。这些神经元的神经丝（NF）的A δ纤维和异凝集素-B4（IB4）的C-纤维的标记物的免疫反应。这些神经元表达神经元型一氧化氮合酶（nNOS）以及与感觉神经元相关的肽，如P物质（SP）和血管活性肠肽（VIP），但不表达降钙素基因相关肽（CGRP）。N-甲基-D-天冬氨酸（NMDA）受体亚单位NR1和NR2D以及蛋白酶激活受体2（PAR 2）也在这些神经元中发现。然而，它们不表达瞬时受体电位受体V1（TRPV 1）或神经激肽1受体（NK 1）。肽和受体的表达表明，至少有两个独立的神经元群体从结肠投射到CNS。这些数据表明，这些结肠脊髓传入神经元（CAN）可能参与伤害性感受。来自CAN的感觉信息是否被动物感知或者是副交感神经反射的一部分目前尚不清楚。(c)2008年IBRO。由爱思唯尔有限公司出版。保留所有权利。
The classification, morphology and function of enteric neurons have been extensively studied in the small and large intestine.. However, little is known about enteric neurons that directly project to the CNS. Previous studies have identified these unique neurons in the rectum, rectospinal neurons, but little was done to characterize them. Therefore, the aim of this study was to identify and characterize enteric neurons in the rat colon that directly project to the CNS by using retrograde neuronal tracing and immunohistochemistry. By applying the retrograde tracers 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (Dil) and Fluorogold (FG) to the L6/S1 segments of the spinal cord, we identified these neurons in both the myenteric and submucosal plexuses of the colon. These neurons were immunoreactive for neurofilament (NF) a marker for A delta-fibers and isolectin-B4 (IB4) a marker for C-fibers. These neurons expressed the enzyme neuronal nitric oxide synthase (nNOS) as well as peptides associated with sensory neurons such as substance P (SP) and vasoactive intestinal polypeptide (VIP) but did not express calcitonin gene-related peptide (CGRP). The N-methyl-D-aspartate (NMDA) receptor subunits NR1 and NR2D and proteinase-activated receptor-2 (PAR2) were also found in these neurons. However they did not express the transient receptor potential receptor V1 (TRPV1) or neurokinin 1 receptor (NK1). The expression of the peptides and receptors suggests that there are at least two separate populations of neurons projecting from the colon to the CNS. The data suggest that these colospinal afferent neurons (CANs) might be involved in nociception. Whether sensory information from CANs is perceived by the animal or is part of the parasympathetic reflex is currently not known. (c) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.