Safety evaluation of probiotic bifidobacteria by analysis of mucin degradation activity and translocation ability
Safety evaluation of probiotic bifidobacteria by analysis of mucin degradation activity and translocation ability
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DOI：
10.1016/j.anaerobe.2009.07.006
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发表时间：
2010-04-01
期刊：
影响因子：
2.3
通讯作者：
Fujisawa, Tomohiko
中科院分区：
文献类型：
--
作者：
Abe, Fumiaki;Muto, Masamichi;Fujisawa, Tomohiko
尽管用于婴儿和幼儿的含有益生菌的营养配方已经变得非常流行，但是已经报道了与益生菌菌株易位相关的一些不利影响。我们通过分析粘蛋白降解活性和易位能力，评估了已用于临床研究并证明具有有益效果的益生菌的安全性。通过液体培养基生长、降解粘蛋白残留物的SDS-PAGE分析和皮氏培养皿降解试验，评价了3株益生双歧杆菌菌株：长双歧杆菌BB 536、短双歧杆菌M-16 V和双歧杆菌M-63的粘蛋白降解活性。所有试验菌株和对照型菌株均不能在含有粘蛋白作为唯一碳源的液体培养基中生长，尽管从粪便样品中获得了良好的生长。在粘蛋白残留的SDS-PAGE分析和琼脂平板上的粘蛋白溶解带的观察中，尽管粪便样品产生阳性结果，但三个测试菌株也没有显示出与模式菌株一样的粘蛋白降解活性。在另一项研究中，高剂量的B。longum B8536口服给药于常规小鼠以检查易位能力。未观察到向血液、肝、脾、肾和肠系膜淋巴结中的移位，未检测到回肠、盲肠和结肠的上皮细胞和粘膜层的紊乱，表明试验菌株不具有移位能力，且未引起肠表面损伤。这些结果解决了当使用益生菌菌株作为益生菌时对细菌易位的担忧，这些益生菌菌株已经在各种临床试验中进行了测试，支持连续使用这些益生菌菌株而不会感到焦虑。(C)2009爱思唯尔有限公司保留所有权利。
Although probiotic-containing nutrient formulas for infants and toddlers have become very popular, some adverse effects related to translocation of probiotic strains have been reported. We assessed the safety of probiotic bifidobacteria that have been used in clinical investigations and proven to have beneficial effects, by analyzing mucin degradation activity and translocation ability. Mucin degradation activities of three probiotic bifidobacteria strains; Bifidobacterium longum BB536, Bifidobacterium breve M-16V and Bifidobacterium infantis M-63, were evaluated by three in vitro tests comprising growth in liquid medium, SDS-PAGE analysis of degraded mucin residues, and degradation assay in Petri dish. All test strains and control type strains failed to grow in the liquid medium containing mucin as the only carbon source, although good growth was obtained from fecal sample. In the SDS-PAGE analyses of mucin residues and observation of mucinolytic zone in agar plate, the three test strains also showed no mucin degradation activity as the type strains, although fecal sample yielded positive results. In another study, a high dose of B. longum B8536 was administered orally to conventional mice to examine the translocation ability. No translocation into blood, liver, spleen, kidney and mesenteric lymph nodes was observed and no disturbance of epithelial cells and mucosal layer in the ileum, cecum and colon was detected, indicating that the test strain had no translocation ability and induced no damage to intestinal surface. These results resolve the concern about bacterial translocation when using bifidobacteria strains as probiotics, which have been tested in various clinical trials, supporting the continuous use of these probiotic strains without anxiety. (C) 2009 Elsevier Ltd. All rights reserved.