Proteins with GGDEF and EAL domains regulate Pseudomonas putida biofilm formation and dispersal
Proteins with GGDEF and EAL domains regulate Pseudomonas putida biofilm formation and dispersal
复制标题
DOI：
10.1111/j.1574-6968.2006.00493.x
复制
发表时间：
2006-12-01
影响因子：
2.1
通讯作者：
Tolker-Nielsen, Tim
中科院分区：
文献类型：
--
作者：
Gjermansen, Morten;Ragas, Paula;Tolker-Nielsen, Tim
微生物生物膜的形成经常在医疗和工业环境中引起问题，并且关于生物膜发展和分散中所涉及的因素的知识对于创建控制该过程的策略是有用的。在这份报告中，我们提出的证据表明，蛋白质与GGDEF和EAL结构域参与调节恶臭假单胞菌生物膜的形成和生物膜的分散。在恶臭假单胞菌中过表达含有GGDEF结构域的大肠杆菌YedQ蛋白导致生物膜形成增加。在恶臭假单胞菌中过量表达E.大肠杆菌YhjH蛋白含有EAL结构域，强烈抑制生物膜的形成。在位于建立的生物膜中的恶臭假单胞菌细胞中诱导YhjH表达导致生物膜的快速分散。这些结果支持了新出现的主题，即GGDEF结构域和EAL结构域蛋白参与调节细菌在漫游生活方式和固着生物膜生活方式之间的转变。
Microbial biofilm formation often causes problems in medical and industrial settings, and knowledge about the factors that are involved in biofilm development and dispersion is useful for creating strategies to control the processes. In this report, we present evidence that proteins with GGDEF and EAL domains are involved in the regulation of biofilm formation and biofilm dispersion in Pseudomonas putida. Overexpression in P. putida of the Escherichia coli YedQ protein, which contains a GGDEF domain, resulted in increased biofilm formation. Overexpression in P. putida of the E. coli YhjH protein, which contains an EAL domain, strongly inhibited biofilm formation. Induction of YhjH expression in P. putida cells situated in established biofilms led to rapid dispersion of the biofilms. These results support the emerging theme that GGDEF-domain and EAL-domain proteins are involved in regulating the transition of bacteria between a roaming lifestyle and a sessile biofilm lifestyle.